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MAP1LC3C Rabbit pAb (A8295)

Datasheet

ABclonal:Western blot - MAP1LC3C Polyclonal Antibody (A8295)

Overview

Product nameMAP1LC3C Rabbit pAb
Catalog No.A8295
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human MAP1LC3C (NP_001004343.1).
SequenceMPPPQKIPSVRPFKQRKSLAIRQEEVAGIRAKFPNKIPVVVERYPRETFLPPLDKTKFLVPQELTMTQFLSIIRSRMVLRATEAFYLLVNNKSLVSMSATMAEIYRDYKDEDGFVYMTYASQETFGCLESAAPRDGSSLEDRPCNPL
Gene ID440738
Swiss protQ9BXW4
SynonymsMAP1LC3C; ATG8J; LC3C
Calculated MW16kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesNCI-H460, OVCAR3, HepG2, 293T, Rat lung
Cellular locationCytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
ABclonal:Western blot - MAP1LC3C Polyclonal Antibody (A8295) }

Western blot - MAP1LC3C Polyclonal Antibody (A8295)

Western blot analysis of extracts of various cell lines, using MAP1LC3C antibody (A8295) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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