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MAP1LC3A Rabbit mAb (A12319)

Datasheet

ABclonal:Western blot - MAP1LC3A Rabbit mAb (A12319)

Western blot analysis of extracts of various cell lines, using MAP1LC3A Rabbit mAb (A12319) at 1：1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - MAP1LC3A Rabbit mAb (A12319)

Western blot analysis of extracts of HeLa cells, using MAP1LC3A Rabbit mAb (A12319) at 1：1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - MAP1LC3A Rabbit mAb (A12319)

Immunohistochemistry of paraffin-embedded human esophageal using MAP1LC3A Rabbit mAb (A12319) at dilution of 1:100 (40x lens).

ABclonal:Immunoprecipitation - MAP1LC3A Rabbit mAb (A12319)

Immunoprecipitation analysis of 300ug extracts of NIH/3T3 cells using 3ug MAP1LC3A antibody (A12319). Western blot was performed from the immunoprecipitate using MAP1LC3A antibody (A12319) at a dilition of 1:1000.NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.

Overview

Product nameMAP1LC3A Rabbit mAb
Catalog No.A12319
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]
ImmunogenA synthesized peptide derived from human MAP1LC3A
SequenceEmail for sequence
Gene ID84557
Swiss protQ9H492
SynonymsATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3
Calculated MW16kDa
Observed MW16KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesHeLa, Mouse brain, Rat brain, NIH/3T3, C6
Cellular locationCytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
ABclonal:Western blot - MAP1LC3A Rabbit mAb (A12319) }

Western blot - MAP1LC3A Rabbit mAb (A12319)

Western blot analysis of extracts of various cell lines, using MAP1LC3A Rabbit mAb (A12319) at 1：1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - MAP1LC3A Rabbit mAb (A12319) }

Western blot - MAP1LC3A Rabbit mAb (A12319)

Western blot analysis of extracts of HeLa cells, using MAP1LC3A Rabbit mAb (A12319) at 1：1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - MAP1LC3A Rabbit mAb (A12319) }

Immunohistochemistry - MAP1LC3A Rabbit mAb (A12319)

Immunohistochemistry of paraffin-embedded human esophageal using MAP1LC3A Rabbit mAb (A12319) at dilution of 1:100 (40x lens).
ABclonal:Immunoprecipitation - MAP1LC3A Rabbit mAb (A12319) }

Immunoprecipitation - MAP1LC3A Rabbit mAb (A12319)

Immunoprecipitation analysis of 300ug extracts of NIH/3T3 cells using 3ug MAP1LC3A antibody (A12319). Western blot was performed from the immunoprecipitate using MAP1LC3A antibody (A12319) at a dilition of 1:1000.NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.

* For research use only. Not for therapeutic or diagnostic purposes.

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