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MAP1LC3A Rabbit pAb (A11438)

Publications (2) Datasheet

ABclonal:Western blot - MAP1LC3A Rabbit pAb (A11438)
ABclonal:Immunohistochemistry - MAP1LC3A Rabbit pAb (A11438)
ABclonal:Immunohistochemistry - MAP1LC3A Rabbit pAb (A11438)

Overview

Product nameMAP1LC3A Rabbit pAb
Catalog No.A11438
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP1LC3A (NP_115903.1).
SequenceMPSDRPFKQRRSFADRCKEVQQIRDQHPSKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELVKIIRRRLQLNPTQAFFLLVNQHSMVSVSTPIADIYE
Gene ID84557
Swiss protQ9H492
SynonymsMAP1LC3A; ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3
Calculated MW14kDa
Observed MW18kDa
ReactivityMouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesMouse brain, Rat heart, Rat brain
Cellular locationCytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
Customer validation

WB(Rattus norvegicus, Mus musculus)

ABclonal:Western blot - MAP1LC3A Rabbit pAb (A11438) }

Western blot - MAP1LC3A Rabbit pAb (A11438)

Western blot analysis of extracts of various cell lines, using MAP1LC3A antibody (A11438) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - MAP1LC3A Rabbit pAb (A11438) }

Immunohistochemistry - MAP1LC3A Rabbit pAb (A11438)

Immunohistochemistry of paraffin-embedded rat brain using MAP1LC3A antibody (A11438) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - MAP1LC3A Rabbit pAb (A11438) }

Immunohistochemistry - MAP1LC3A Rabbit pAb (A11438)

Immunohistochemistry of paraffin-embedded mouse spinal cord using MAP1LC3A antibody (A11438) at dilution of 1:100 (40x lens).

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