Western blot analysis of extracts of various cell lines, using MAP1LC3A antibody (A11438) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using MAP1LC3A antibody (A11438) at dilution of 1:100 (40x lens).
| Product name | MAP1LC3A Rabbit pAb |
|---|---|
| Catalog No. | A11438 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP1LC3A (NP_115903.1). |
|---|---|
| Sequence | MPSDRPFKQRRSFADRCKEVQQIRDQHPSKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELVKIIRRRLQLNPTQAFFLLVNQHSMVSVSTPIADIYE |
| Gene ID | 84557 |
| Swiss prot | Q9H492 |
| Synonyms | MAP1LC3A; ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3 |
| Calculated MW | 14kDa |
| Observed MW | 18kDa |
| Reactivity | Mouse, Rat |
|---|---|
| Tested applications | WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry |
| Positive samples | Mouse brain, Rat heart, Rat brain |
| Cellular location | Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton |
| Customer validation | WB(Mus musculus, Rattus norvegicus) |
* For research use only. Not for therapeutic or diagnostic purposes.
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