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Lipocalin-2/NGAL Rabbit pAb (A2092)

Review (1)Publications (5) Datasheet

ABclonal:Western blot - Lipocalin-2/NGAL Rabbit pAb (A2092)

Western blot analysis of extracts of various cell lines, using Lipocalin-2/NGAL antibody (A2092) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - Lipocalin-2/NGAL Rabbit pAb (A2092)

Immunofluorescence analysis of mouse kidney using Lipocalin-2/NGAL antibody (A2092) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Lipocalin-2/NGAL Rabbit pAb(A2092)

Overview

Product nameLipocalin-2/NGAL Rabbit pAb
Catalog No.A2092
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 21-198 of human Lipocalin-2/NGAL (NP_005555.2).
SequenceQDSTSDLIPAPPLSKVPLQQNFQDNQFQGKWYVVGLAGNAILREDKDPQKMYATIYELKEDKSYNVTSVLFRKKKCDYWIRTFVPGCQPGEFTLGNIKSYPGLTSYLVRVVSTNYNQHAMVFFKKVSQNREYFKITLYGRTKELTSELKENFIRFSKSLGLPENHIVFPVPIDQCIDG
Gene ID3934
Swiss protP80188
SynonymsLCN2; 24p3; MSFI; NGAL; p25
Calculated MW22kDa
Observed MW22kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesDU145, HT-29, BxPC-3, Mouse spleen
Cellular locationSecreted
Customer validation

(Mouse kidney)

WB(Mus musculus, Rattus norvegicus)

IF(Mus musculus)

IF(Homo sapiens)

ABclonal:Western blot - Lipocalin-2/NGAL Rabbit pAb (A2092) }

Western blot - Lipocalin-2/NGAL Rabbit pAb (A2092)

Western blot analysis of extracts of various cell lines, using Lipocalin-2/NGAL antibody (A2092) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - Lipocalin-2/NGAL Rabbit pAb (A2092) }

Immunofluorescence - Lipocalin-2/NGAL Rabbit pAb (A2092)

Immunofluorescence analysis of mouse kidney using Lipocalin-2/NGAL antibody (A2092) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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