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Lamin A/C Rabbit pAb (A0249)

Reviews (4)Publications (17) Datasheet

ABclonal:Western blot - Lamin A/C Rabbit pAb (A0249)
ABclonal:Western blot - Lamin A/C Rabbit pAb (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Rabbit pAb (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Rabbit pAb (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Rabbit pAb (A0249)
ABclonal:Immunofluorescence - Lamin A/C Rabbit pAb (A0249)
Review
ABclonal: review for Lamin A/C Rabbit pAb(A0249)
Review
ABclonal: review for Lamin A/C Rabbit pAb(A0249)
Review
ABclonal: review for Lamin A/C Rabbit pAb(A0249)
Review
ABclonal: review for Lamin A/C Rabbit pAb(A0249)

Overview

Product nameLamin A/C Rabbit pAb
Catalog No.A0249
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin C (NP_005563.1).
SequenceSSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQNTWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHVSGSRR
Gene ID4000
Swiss protP02545
SynonymsLMNA; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; MADA; PRO1; lamin; Lamin A/C
Calculated MW62-74kDa
Observed MW63KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, K-562, A-549
Cellular locationNucleus, Nucleus envelope, Nucleus lamina, Nucleus speckle, nucleoplasm
Customer validation

(Primary mouse liver, Mouse splenocytes, Human cells, U251)

WB(Homo sapiens, Mus musculus)

ELISA(Mus musculus)

IF(Homo sapiens)

ABclonal:Western blot - Lamin A/C Rabbit pAb (A0249) }

Western blot - Lamin A/C Rabbit pAb (A0249)

Western blot analysis of extracts of various cell lines, using Lamin A/C Rabbit pAb (A0249) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Lamin A/C Rabbit pAb (A0249) }

Western blot - Lamin A/C Rabbit pAb (A0249)

Western blot analysis of extracts from normal (control) and Lamin A/C Rabbit pAb knockout (KO) HeLa cells, using Lamin A/C Rabbit pAb antibody (A0249) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Lamin A/C Rabbit pAb (A0249) }

Immunohistochemistry - Lamin A/C Rabbit pAb (A0249)

Immunohistochemistry of paraffin-embedded rat brain using Lamin A/C antibody (A0249) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Lamin A/C Rabbit pAb (A0249) }

Immunohistochemistry - Lamin A/C Rabbit pAb (A0249)

Immunohistochemistry of paraffin-embedded mouse kidney using Lamin A/C antibody (A0249) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Lamin A/C Rabbit pAb (A0249) }

Immunohistochemistry - Lamin A/C Rabbit pAb (A0249)

Immunohistochemistry of paraffin-embedded Human gastric cancer using Lamin A/C antibody (A0249) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Lamin A/C Rabbit pAb (A0249) }

Immunofluorescence - Lamin A/C Rabbit pAb (A0249)

Confocal immunofluorescence analysis of A-431 cells using Lamin A/C Polyclonal Antibody (A0249) at dilution of 1:200. Blue: DAPI for nuclear staining.

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