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Lamin A/C Polyclonal Antibody (A0249)

Review (4)Publication (12) Datasheet

ABclonal:Western blot - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249)
ABclonal:Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249)
Review
ABclonal: review for Lamin A/C Polyclonal Antibody(A0249)
Review
ABclonal: review for Lamin A/C Polyclonal Antibody(A0249)
Review
ABclonal: review for Lamin A/C Polyclonal Antibody(A0249)
Review
ABclonal: review for Lamin A/C Polyclonal Antibody(A0249)

Overview

Product nameLamin A/C Polyclonal Antibody
Catalog No.A0249
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin C (NP_005563.1).
SequenceSSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQNTWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHVSGSRR
Gene ID4000
Swiss protP02545
SynonymsLMNA; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; MADA; PRO1; lamin
Calculated MW62-74kDa
Observed MW69kDa, 72kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, C6
Cellular locationNucleus, Nucleus envelope, Nucleus lamina, Nucleus speckle, nucleoplasm
Customer validation

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WB(Human, Mouse)

ELISA(Mouse)

ABclonal:Western blot - Lamin A/C Polyclonal Antibody (A0249) }

Western blot - Lamin A/C Polyclonal Antibody (A0249)

Western blot analysis of extracts of various cell lines, using Lamin A/C antibody (A0249) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours or treated by Paclitaxel (100 nM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded rat brain using Lamin A/C antibody (A0249) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded mouse kidney using Lamin A/C antibody (A0249) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249) }

Immunohistochemistry - Lamin A/C Polyclonal Antibody (A0249)

Immunohistochemistry of paraffin-embedded Human gastric cancer using Lamin A/C antibody (A0249) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249) }

Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249)

Immunofluorescence analysis of HeLa cells using Lamin A/C antibody (A0249). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249) }

Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249)

Confocal immunofluorescence analysis of HeLa cells using Lamin A/C Polyclonal Antibody (A0249) at dilution of 1:200. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249) }

Immunofluorescence - Lamin A/C Polyclonal Antibody (A0249)

Confocal immunofluorescence analysis of U-2 OS cells using Lamin A/C Polyclonal Antibody (A0249) at dilution of 1:200. Blue: DAPI for nuclear staining.

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