Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Lamin A/C Mouse mAb (A11801)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - Lamin A/C Mouse mAb (A11801)

Western blot analysis of various lysates using Lamin A/C Mouse mAb (A11801) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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Overview

Product name Lamin A/C Mouse mAb
Catalog No. A11801
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene is part of the nuclear lamina, a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.

Immunogen information

Immunogen Recombinant protein of human LMNA
Sequence Email for sequence
Gene ID 4000
Swiss prot P02545
Synonyms FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B; Lamin A/C
Calculated MW 74kDa
Observed MW 69kDa, 78kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples K562, HeLa, SKOV3, BT474
Cellular location Nucleus, Nucleus envelope, Nucleus lamina, Nucleus speckle, nucleoplasm

Documents

Certificate of Compliance

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Lot number

Research Area

Lamin A/C Mouse mAb images

ABclonal:Western blot - Lamin A/C Mouse mAb (A11801)}

Western blot - Lamin A/C Mouse mAb (A11801)

Western blot analysis of various lysates using Lamin A/C Mouse mAb (A11801) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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Related Targets

Based on existing publications, the following genes are closely related to research on LMNA. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to LMNA. (Distance between topics and target gene indicate popularity.) LMNA

* Data provided by citexs.com, for reference only.

Publishing research using A11801? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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