Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

LZTS1 Rabbit pAb (A16496)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - LZTS1 Rabbit pAb (A16496)

Western blot analysis of lysates from Rat brain, using LZTS1 Rabbit pAb (A16496) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Western blot - LZTS1 Rabbit pAb (A16496)

Western blot analysis of various lysates using LZTS1 Rabbit pAb (A16496) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name LZTS1 Rabbit pAb
Catalog No. A16496
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a tumor suppressor protein that is ubiquitously expressed in normal tissues. In uveal melanomas, expression of this protein is silenced in rapidly metastasizing and metastatic tumor cells but has normal expression in slowly metastasizing or nonmetastasizing tumor cells. This protein may have a role in cell-cycle control by interacting with the Cdk1/cyclinB1 complex. This gene is located on chromosomal region 8p22. Loss of heterozygosity (LOH) in the 8p arm is a common characteristic of many types of cancer.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 337-596 of human LZTS1 (NP_066300.1).
Sequence QLQQEKRQLRQELESLMKEQDLLETKLRSYEREKTSFGPALEETQWEVCQKSGEISLLKQQLKESQTEVNAKASEILGLKAQLKDTRGKLEGLELRTQDLEGALRTKGLELEVCENELQRKKNEAELLREKVNLLEQELQELRAQAALARDMGPPTFPEDVPALQRELERLRAELREERQGHDQMSSGFQHERLVWKEEKEKVIQYQKQLQQSYVAMYQRNQRLEKALQQLARGDSAGEPLEVDLEGADIPYEDIIATEI
Gene ID 11178
Swiss prot Q9Y250
Synonyms F37; FEZ1; LZTS1
Calculated MW 67kDa
Observed MW 67kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:5000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Jurkat, Mouse brain, Rat brain
Cellular location Cell junction, Cell membrane, Cell projection, Cytoplasm, dendritic spine, postsynaptic cell membrane, postsynaptic density, synapse

Research Area

LZTS1 Rabbit pAb images

ABclonal:Western blot - LZTS1 Rabbit pAb (A16496)}

Western blot - LZTS1 Rabbit pAb (A16496)

Western blot analysis of lysates from Rat brain, using LZTS1 Rabbit pAb (A16496) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Western blot - LZTS1 Rabbit pAb (A16496)}

Western blot - LZTS1 Rabbit pAb (A16496)

Western blot analysis of various lysates using LZTS1 Rabbit pAb (A16496) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on LZTS1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to LZTS1. (Distance between topics and target gene indicate popularity.) LZTS1

* Data provided by citexs.com, for reference only.

Publishing research using A16496? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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