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LRP5 Rabbit pAb (A0130)

Publications (4) Datasheet

ABclonal:Western blot - LRP5 Rabbit pAb (A0130)

Western blot analysis of extracts of various cell lines, using LRP5 antibody (A0130) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - LRP5 Rabbit pAb (A0130)

Immunohistochemistry of paraffin-embedded human liver using LRP5 antibody (A0130) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - LRP5 Rabbit pAb (A0130)

Immunohistochemistry of paraffin-embedded human prostate using LRP5 antibody (A0130) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - LRP5 Rabbit pAb (A0130)

Immunohistochemistry of paraffin-embedded mouse lung using LRP5 antibody (A0130) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - LRP5 Rabbit pAb (A0130)

Immunofluorescence analysis of NIH/3T3 cells using LRP5 Rabbit pAb (A0130) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - LRP5 Rabbit pAb (A0130)

Immunofluorescence analysis of PC-12 cells using LRP5 Rabbit pAb (A0130) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameLRP5 Rabbit pAb
Catalog No.A0130
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transmembrane low-density lipoprotein receptor that binds and internalizes ligands in the process of receptor-mediated endocytosis. This protein also acts as a co-receptor with Frizzled protein family members for transducing signals by Wnt proteins and was originally cloned on the basis of its association with type 1 diabetes mellitus in humans. This protein plays a key role in skeletal homeostasis and many bone density related diseases are caused by mutations in this gene. Mutations in this gene also cause familial exudative vitreoretinopathy. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1000-1250 of human LRP5 (NP_002326.2).
SequenceIKRAKDDGTQPFVLTSLSQGQNPDRQPHDLSIDIYSRTLFWTCEATNTINVHRLSGEAMGVVLRGDRDKPRAIVVNAERGYLYFTNMQDRAAKIERAALDGTEREVLFTTGLIRPVALVVDNTLGKLFWVDADLKRIESCDLSGANRLTLEDANIVQPLGLTILGKHLYWIDRQQQMIERVEKTTGDKRTRIQGRVAHLTGIHAVEEVSLEEFSAHPCARDNGGCSHICIAKGDGTPRCSCPVHLVLLQNL
Gene ID4041
Swiss protO75197
SynonymsLRP5; BMND1; EVR1; EVR4; HBM; LR3; LRP-5; LRP7; OPPG; OPS; OPTA1; VBCH2
Calculated MW179kDa
Observed MW180-200KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse liver, Rat liver
Cellular locationEndoplasmic reticulum, Membrane, Single-pass type I membrane protein
Customer validation

WB(Rattus norvegicus, Mus musculus)

ABclonal:Western blot - LRP5 Rabbit pAb (A0130) }

Western blot - LRP5 Rabbit pAb (A0130)

Western blot analysis of extracts of various cell lines, using LRP5 antibody (A0130) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - LRP5 Rabbit pAb (A0130) }

Immunohistochemistry - LRP5 Rabbit pAb (A0130)

Immunohistochemistry of paraffin-embedded human liver using LRP5 antibody (A0130) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - LRP5 Rabbit pAb (A0130) }

Immunohistochemistry - LRP5 Rabbit pAb (A0130)

Immunohistochemistry of paraffin-embedded human prostate using LRP5 antibody (A0130) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - LRP5 Rabbit pAb (A0130) }

Immunohistochemistry - LRP5 Rabbit pAb (A0130)

Immunohistochemistry of paraffin-embedded mouse lung using LRP5 antibody (A0130) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - LRP5 Rabbit pAb (A0130) }

Immunofluorescence - LRP5 Rabbit pAb (A0130)

Immunofluorescence analysis of NIH/3T3 cells using LRP5 Rabbit pAb (A0130) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - LRP5 Rabbit pAb (A0130) }

Immunofluorescence - LRP5 Rabbit pAb (A0130)

Immunofluorescence analysis of PC-12 cells using LRP5 Rabbit pAb (A0130) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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