Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

LRP2 Rabbit pAb (A3612)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Mouse, Rat

ABclonal:Western blot - LRP2 Rabbit pAb (A3612)

Western blot analysis of extracts of mouse lung, using LRP2 antibody (A3612) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - LRP2 Rabbit pAb (A3612)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using LRP2 Rabbit pAb (A3612) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - LRP2 Rabbit pAb (A3612)

Immunohistochemistry analysis of paraffin-embedded rat kidney using LRP2 Rabbit pAb (A3612) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Overview

Product name LRP2 Rabbit pAb
Catalog No. A3612
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 4446-4655 of human LRP2 (NP_004516.2).
Sequence FHYRRTGSLLPALPKLPSLSSLVKPSENGNGVTFRSGADLNMDIGVSGFGPETAIDRSMAMSEDFVMEMGKQPIIFENPMYSARDSAVKVVQPIQVTVSENVDNKNYGSPINPSEIVPETNPTSPAADGTQVTKWNLFKRKSKQTTNFENPIYAQMENEQKESVAATPPPSPSLPAKPKPPSRRDPTPTYSATEDTFKDTANLVKEDSEV
Gene ID 4036
Swiss prot P98164
Synonyms DBS; GP330; LRP-2; LRP2
Calculated MW 522kDa
Observed MW 522kDa

Applications

Reactivity Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:200 - 1:1000
  • IHC-P 1:20 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples Mouse lung
Cellular location Membrane, Single-pass type I membrane protein, coated pit

Documents

Certificate of Compliance

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Research Area

LRP2 Rabbit pAb images

ABclonal:Western blot - LRP2 Rabbit pAb (A3612)}

Western blot - LRP2 Rabbit pAb (A3612)

Western blot analysis of extracts of mouse lung, using LRP2 antibody (A3612) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - LRP2 Rabbit pAb (A3612)}

Immunohistochemistry - LRP2 Rabbit pAb (A3612)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using LRP2 Rabbit pAb (A3612) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - LRP2 Rabbit pAb (A3612)}

Immunohistochemistry - LRP2 Rabbit pAb (A3612)

Immunohistochemistry analysis of paraffin-embedded rat kidney using LRP2 Rabbit pAb (A3612) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on LRP2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to LRP2. (Distance between topics and target gene indicate popularity.) LRP2

* Data provided by citexs.com, for reference only.

Publishing research using A3612? Please let us know so that we can cite the reference in this datasheet.

ELISA Kits (1)

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Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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