Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

L-Plastin/LCP1 Rabbit pAb (A5561)

Publication (1) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - L-Plastin/LCP1 Rabbit pAb (A5561)

Western blot analysis of various lysates using L-Plastin/LCP1 Rabbit pAb (A5561) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunoprecipitation - L-Plastin/LCP1 Rabbit pAb (A5561)

Immunoprecipitation analysis of 150 μg extracts of Jurkat cells using 3 μg L-Plastin/LCP1 antibody (A5561). Western blot was performed from the immunoprecipitate using L-Plastin/LCP1 antibody (A5561) at a dilution of 1:1000.

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Overview

Product name L-Plastin/LCP1 Rabbit pAb
Catalog No. A5561
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human L-Plastin/LCP1 (NP_002289.2).
Sequence MARGSVSDEEMMELREAFAKVDTDGNGYISFNELNDLFKAACLPLPGYRVREITENLMATGDLDQDGRISFDEFIKIFHGLKSTDVAKTFRKAINKKEGICAIGGTSEQSSVGTQHSYSEEEKYAFVNWINKALENDPDCRHVIPMNPNTNDLFNAVGDGIVLCKMINLSVPDTIDERTINKKKLTPFTIQENLNLALNSASAIGCHVVNIGAEDLKEGKPYLVLGLLWQVIKIGLFADIELSRNEALIALLREGESLEDLMKLSPEELL
Gene ID 3936
Swiss prot P13796
Synonyms LPL; CP64; PLS2; LC64P; HEL-S-37; L-PLASTIN; L-Plastin/LCP1
Calculated MW 70kDa
Observed MW 70kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunoprecipitation    
Positive samples Raji, K-562, DU145, Jurkat, Mouse thymus, Mouse spleen
Cellular location Cell junction, Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cytoskeleton, ruffle membrane
Customer validation

WB (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

L-Plastin/LCP1 Rabbit pAb images

ABclonal:Western blot - L-Plastin/LCP1 Rabbit pAb (A5561)}

Western blot - L-Plastin/LCP1 Rabbit pAb (A5561)

Western blot analysis of various lysates using L-Plastin/LCP1 Rabbit pAb (A5561) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunoprecipitation - L-Plastin/LCP1 Rabbit pAb (A5561)}

Immunoprecipitation - L-Plastin/LCP1 Rabbit pAb (A5561)

Immunoprecipitation analysis of 150 μg extracts of Jurkat cells using 3 μg L-Plastin/LCP1 antibody (A5561). Western blot was performed from the immunoprecipitate using L-Plastin/LCP1 antibody (A5561) at a dilution of 1:1000.

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Related Targets

Based on existing publications, the following genes are closely related to research on LCP1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to LCP1. (Distance between topics and target gene indicate popularity.) LCP1

* Data provided by citexs.com, for reference only.

Publishing research using A5561? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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