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LPCAT2 Polyclonal Antibody (A4805)

Datasheet

ABclonal:Western blot - LPCAT2 Polyclonal Antibody (A4805)

Overview

Product nameLPCAT2 Polyclonal Antibody
Catalog No.A4805
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the lysophospholipid acyltransferase family. The encoded enzyme may function in two ways: to catalyze the biosynthesis of platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) from 1-O-alkyl-sn-glycero-3-phosphocholine, and to catalyze the synthesis of glycerophospholipid precursors from arachidonyl-CoA and lysophosphatidylcholine. The encoded protein may function in membrane biogenesis and production of platelet-activating factor in inflammatory cells. The enzyme may localize to the endoplasmic reticulum and the Golgi.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 160-544 of human LPCAT2 (NP_060309.2).
SequenceLPSMVSRNENAQVPLIGRLLRAVQPVLVSRVDPDSRKNTINEIIKRTTSGGEWPQILVFPEGTCTNRSCLITFKPGAFIPGVPVQPVLLRYPNKLDTVTWTWQGYTFIQLCMLTFCQLFTKVEVEFMPVQVPNDEEKNDPVLFANKVRNLMAEALGIPVTDHTYEDCRLMISAGQLTLPMEAGLVEFTKISRKLKLDWDGVRKHLDEYASIASSSKGGRIGIEEFAKYLKLPVSDVLRQLFALFDRNHDGSIDFREYVIGLAVLCNPSNTEEIIQVAFKLFDVDEDGYITEEEFSTILQASLGVPDLDVSGLFKEIAQGDSISYEEFKSFALKHPEYAKIFTTYLDLQTCHVFSLPKEVQTTPSTASNKVSPEKHEESTSDKKDD
Gene ID54947
Swiss protQ7L5N7
SynonymsLPCAT2; AGPAT11; AYTL1; LysoPAFAT; lysophosphatidylcholine acyltransferase 2
Calculated MW30kDa/60kDa
Observed MW52kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, Jurkat
Cellular locationEndoplasmic reticulum membrane, Golgi apparatus membrane, Lipid droplet, Single-pass type II membrane protein
ABclonal:Western blot - LPCAT2 Polyclonal Antibody (A4805) }

Western blot - LPCAT2 Polyclonal Antibody (A4805)

Western blot analysis of extracts of various cell lines, using LPCAT2 antibody (A4805) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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