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LGR5/GPR49 Rabbit pAb (A10545)

Review (1)Publications (2) Datasheet

ABclonal:Western blot - LGR5/GPR49 Rabbit pAb (A10545)

Western blot analysis of extracts of various cell lines, using LGR5 antibody (A10545) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - LGR5/GPR49 Rabbit pAb (A10545)

Western blot analysis of extracts of various cell lines, using LGR5 antibody (A10545) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - LGR5/GPR49 Rabbit pAb (A10545)

Immunohistochemistry of paraffin-embedded human colon carcinoma using LGR5/GPR49 Rabbit pAb (A10545) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - LGR5/GPR49 Rabbit pAb (A10545)

Immunohistochemistry of paraffin-embedded human oophoroma using LGR5/GPR49 Rabbit pAb (A10545) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - LGR5/GPR49 Rabbit pAb (A10545)

Immunofluorescence analysis of NIH/3T3 cells using LGR5/GPR49 antibody (A10545) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - LGR5/GPR49 Rabbit pAb (A10545)

Immunofluorescence analysis of PC12 cells using LGR5/GPR49 antibody (A10545) at dilution of 1:100.

Review
ABclonal: review for LGR5/GPR49 Rabbit pAb(A10545)

Overview

Product nameLGR5/GPR49 Rabbit pAb
Catalog No.A10545
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 824-907 of human LGR5/GPR49 (NP_003658.1).
SequenceNPHFKEDLVSLRKQTYVWTRSKHPSLMSINSDDVEKQSCDSTQALVTFTSSSITYDLPPSSVPSPAYPVTESCHLSSVAFVPCL
Gene ID8549
Swiss protO75473
SynonymsLGR5; FEX; GPR49; GPR67; GRP49; HG38
Calculated MW92kDa/97kDa/99kDa
Observed MW100KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:1000 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHT-29, HepG2, SH-SY5Y, Mouse brain, Rat brain
Cellular locationCell membrane, Golgi apparatus, Multi-pass membrane protein, trans-Golgi network membrane
Customer validation

(Mouse heart tissue)

WB(Homo sapiens)

ABclonal:Western blot - LGR5/GPR49 Rabbit pAb (A10545) }

Western blot - LGR5/GPR49 Rabbit pAb (A10545)

Western blot analysis of extracts of various cell lines, using LGR5 antibody (A10545) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - LGR5/GPR49 Rabbit pAb (A10545) }

Western blot - LGR5/GPR49 Rabbit pAb (A10545)

Western blot analysis of extracts of various cell lines, using LGR5 antibody (A10545) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - LGR5/GPR49 Rabbit pAb (A10545) }

Immunohistochemistry - LGR5/GPR49 Rabbit pAb (A10545)

Immunohistochemistry of paraffin-embedded human colon carcinoma using LGR5/GPR49 Rabbit pAb (A10545) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - LGR5/GPR49 Rabbit pAb (A10545) }

Immunohistochemistry - LGR5/GPR49 Rabbit pAb (A10545)

Immunohistochemistry of paraffin-embedded human oophoroma using LGR5/GPR49 Rabbit pAb (A10545) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - LGR5/GPR49 Rabbit pAb (A10545) }

Immunofluorescence - LGR5/GPR49 Rabbit pAb (A10545)

Immunofluorescence analysis of NIH/3T3 cells using LGR5/GPR49 antibody (A10545) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - LGR5/GPR49 Rabbit pAb (A10545) }

Immunofluorescence - LGR5/GPR49 Rabbit pAb (A10545)

Immunofluorescence analysis of PC12 cells using LGR5/GPR49 antibody (A10545) at dilution of 1:100.

* For research use only. Not for therapeutic or diagnostic purposes.

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