Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

LEFTY1/2 Rabbit mAb (A2387)

Datasheet SDS COA

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - LEFTY1/2 Rabbit mAb (A2387)

Western blot analysis of extracts of various cell lines, using LEFTY1/2 Rabbit mAb (A2387) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - LEFTY1/2 Rabbit mAb (A2387)

Western blot analysis of extracts of various cell lines, using LEFTY1/2 Rabbit mAb (A2387) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - LEFTY1/2 Rabbit mAb (A2387)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using LEFTY1/2 Rabbit mAb (A2387) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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    Overview

    Product nameLEFTY1/2 Rabbit mAb
    Catalog No.A2387
    Host speciesRabbit
    Purification methodAffinity purification
    IsotypeIgG
    CloneNo.ARC2149

    Background

    This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. This gene is closely linked to both a related family member and a related pseudogene. [provided by RefSeq, Aug 2016]

    Immunogen information

    ImmunogenA synthetic peptide corresponding to a sequence within amino acids 267-366 of human LEFTY1/2 (O75610).
    SequenceEMYIDLQGMKWAENWVLEPPGFLAYECVGTCRQPPEALAFKWPFLGPRQCIASETDSLPMIVSIKEGGRTRPQVVSLPNMRVQKCSCASDGALVPRRLQP
    Gene ID106377044
    Swiss protO75610O00292
    SynonymsLEFTB; LEFTYB
    Calculated MW34.44kDa
    Observed MW41kDa

    Applications

    ReactivityHuman, Mouse, Rat
    Tested applicationsTesting results
    IHC-PHuman
    WBHumanMouseRat
    Recommended dilution
    • WB 1:500 - 1:2000
    • IHC-P 1:50 - 1:200
    Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
    Application keyWestern blotting    Immunohistochemistry    
    Positive samplesHT-29, BxPC-3, Mouse brain, Rat brain
    Cellular locationExtracellular space

    Documents

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    Lot number

    LEFTY1/2 Rabbit mAb images

    ABclonal:Western blot - LEFTY1/2 Rabbit mAb (A2387)}

    Western blot - LEFTY1/2 Rabbit mAb (A2387)

    Western blot analysis of extracts of various cell lines, using LEFTY1/2 Rabbit mAb (A2387) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - LEFTY1/2 Rabbit mAb (A2387)}

    Western blot - LEFTY1/2 Rabbit mAb (A2387)

    Western blot analysis of extracts of various cell lines, using LEFTY1/2 Rabbit mAb (A2387) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - LEFTY1/2 Rabbit mAb (A2387)}

    Immunohistochemistry - LEFTY1/2 Rabbit mAb (A2387)

    Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using LEFTY1/2 Rabbit mAb (A2387) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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    Alternatively, call us at 888.754.5670.

    Related Targets

    Based on existing publications, the following genes are closely related to research on LEFTY1. (Numbers indicate number of published articles where both targets appear.)

    ◀1/▶

    Related Research Area

    Based on existing publications, the following research topics are related to LEFTY1. (Distance between topics and target gene indicate popularity.) LEFTY1

    * Data provided by citexs.com, for reference only.

    Publishing research using A2387? Please let us know so that we can cite the reference in this datasheet.

    * For research use only. Not for therapeutic or diagnostic purposes.

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