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LC3A/LC3B Rabbit pAb (A5618)

Publications (8) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - LC3A/LC3B Rabbit pAb (A5618)

Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody (A5618) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Western blot - LC3A/LC3B Rabbit pAb (A5618)

Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody (A5618) at 1:500 dilution.293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.C6 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

Overview

Product nameLC3A/LC3B Rabbit pAb
Catalog No.A5618
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-60 of human LC3A (NP_115903.1).
SequenceMPSDRPFKQRRSFADRCKEVQQIRDQHPSKIPVIIERYKGEKQLPVLDKTKFLVPDHVNM
Gene ID8455781631
Swiss protQ9GZQ8Q9H492
SynonymsMAP1LC3B; ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a; MAP1LC3A; ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3; LC3A/LC3B
Calculated MW14kDa
Observed MW14kDa/16kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, SH-SY5Y, A375, Mouse brain, Rat brain, NIH/3T3, C6
Cellular locationCytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
Customer validation

WB(Gallus gallus, Rattus norvegicus, Homo sapiens, Mus musculus, Pollen)

IF(Pollen)

    ABclonal:Western blot - LC3A/LC3B Rabbit pAb (A5618) }

    Western blot - LC3A/LC3B Rabbit pAb (A5618)

    Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody (A5618) at 1:500 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 20s.
    ABclonal:Western blot - LC3A/LC3B Rabbit pAb (A5618) }

    Western blot - LC3A/LC3B Rabbit pAb (A5618)

    Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody (A5618) at 1:500 dilution.293T cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.NIH/3T3 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.C6 cells were treated by Chloroquine (50 μM) at 37℃ for 20 hours.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% BSA.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 3min.

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