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LAMP1 Rabbit pAb (A2582)

Review (1)Publications (3) Datasheet

ABclonal:Western blot - LAMP1 Polyclonal Antibody (A2582)
ABclonal:Immunofluorescence - LAMP1 Polyclonal Antibody (A2582)
ABclonal:Immunofluorescence - LAMP1 Polyclonal Antibody (A2582)
Review
ABclonal: review for LAMP1 Rabbit pAb(A2582)

Overview

Product nameLAMP1 Rabbit pAb
Catalog No.A2582
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 80-280 of human LAMP1 (NP_005552.3).
SequenceCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHS
Gene ID3916
Swiss protP11279
SynonymsLAMP1; CD107a; LAMPA; LGP120
Calculated MW38kDa/44kDa
Observed MW120kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHT-29, 293T, BT-474
Cellular locationCell membrane, Endosome membrane, Late endosome, Lysosome membrane, Single-pass type I membrane protein
Customer validation

(HEK293T)

WB(Homo sapiens, Mus musculus)

IF(Homo sapiens)

IHC(Mus musculus)

ABclonal:Western blot - LAMP1 Polyclonal Antibody (A2582) }

Western blot - LAMP1 Polyclonal Antibody (A2582)

Western blot analysis of extracts of various cell lines, using MCL1 antibody (A2582) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - LAMP1 Polyclonal Antibody (A2582) }

Immunofluorescence - LAMP1 Polyclonal Antibody (A2582)

Immunofluorescence analysis of HeLa cells using LAMP1 antibody (A2582) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - LAMP1 Polyclonal Antibody (A2582) }

Immunofluorescence - LAMP1 Polyclonal Antibody (A2582)

Immunofluorescence analysis of NIH/3T3 cells using LAMP1 antibody (A2582) at dilution of 1:100. Blue: DAPI for nuclear staining.

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