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Ki67 Rabbit pAb (A2094)

Publications (37) Datasheet

ABclonal:Ki67 Rabbit pAb
ABclonal:Western blot - Ki67 Rabbit pAb (A2094)

Western blot analysis of extracts of various cell lines, using Ki67 Rabbit pAb (A2094) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Ki67 Rabbit pAb (A2094)

Immunohistochemistry of paraffin-embedded human lung cancer using Ki67 antibody (A2094) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - Ki67 Rabbit pAb (A2094)

Immunofluorescence analysis of HeLa cells using Ki67 antibody (A2094) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameKi67 Rabbit pAb
Catalog No.A2094
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1200-1300 of human Ki67 (NP_001139438.1).
SequenceQKLDLTENLTGSKRRLQTPKEKAQALEDLAGFKELFQTRGHTEESMTNDKTAKVACKSSQPDPDKNPASSKRRLKTSLGKVGVKEELLAVGKLTQTSGETT
Gene ID4288
Swiss protP46013
SynonymsMKI67; KIA; MIB-; MIB-1; PPP1R105; marker of proliferation Ki-67; Ki67
Calculated MW319kDa/358kDa
Observed MW359KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat, U-251MG, Mouse testis
Cellular locationChromosome, Nucleus, nucleolus
Customer validation

WB(Mus musculus, Homo sapiens, Rattus norvegicus)

IF(Homo sapiens, Mus musculus)

IHC(Homo sapiens, Mus musculus, Rattus norvegicus)

IF(Homo sapiens, Rattus norvegicus)

FC(Mus musculus)

ABclonal:Western blot - Ki67 Rabbit pAb (A2094) }

Western blot - Ki67 Rabbit pAb (A2094)

Western blot analysis of extracts of various cell lines, using Ki67 Rabbit pAb (A2094) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Ki67 Rabbit pAb (A2094) }

Immunohistochemistry - Ki67 Rabbit pAb (A2094)

Immunohistochemistry of paraffin-embedded human lung cancer using Ki67 antibody (A2094) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - Ki67 Rabbit pAb (A2094) }

Immunofluorescence - Ki67 Rabbit pAb (A2094)

Immunofluorescence analysis of HeLa cells using Ki67 antibody (A2094) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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