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[KO Validated] p53 Rabbit mAb (A19585)

Publications (2) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Rat

ABclonal:Western blot - [KO Validated] p53 Rabbit mAb (A19585)

Western blot analysis of extracts from normal (control) and p53 knockout (KO) 293T cells, using p53 antibody (A19585) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] p53 Rabbit mAb (A19585)

Immunohistochemistry of paraffin-embedded Human colon (negative control sample) using [KO Validated] p53 Rabbit mAb (A19585) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] p53 Rabbit mAb (A19585)

Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] p53 Rabbit mAb (A19585) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] p53 Rabbit mAb (A19585)

Immunohistochemistry of paraffin-embedded Human hepatocholangiocarcinoma using [KO Validated] p53 Rabbit mAb (A19585) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] p53 Rabbit mAb (A19585)

Immunohistochemistry of paraffin-embedded Human liver (negative control sample) using [KO Validated] p53 Rabbit mAb (A19585) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunoprecipitation - [KO Validated] p53 Rabbit mAb (A19585)

Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug p53 antibody (A19585). Western blot was performed from the immunoprecipitate using p53 antibody (A19585) at a dilition of 1:1000.

Overview

Product name[KO Validated] p53 Rabbit mAb
Catalog No.A19585
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-393 of human p53 (NP_000537.3).
SequenceMEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD
Gene ID7157
Swiss protP04637
SynonymsBCC7; LFS1; P53; TRP53; p53; TP53; BMFS5
Calculated MW53kDa
Observed MW52KDa
ReactivityHuman, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samples293T
Cellular locationCytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - [KO Validated] p53 Rabbit mAb (A19585) }

Western blot - [KO Validated] p53 Rabbit mAb (A19585)

Western blot analysis of extracts from normal (control) and p53 knockout (KO) 293T cells, using p53 antibody (A19585) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] p53 Rabbit mAb (A19585) }

Immunohistochemistry - [KO Validated] p53 Rabbit mAb (A19585)

Immunohistochemistry of paraffin-embedded Human colon (negative control sample) using [KO Validated] p53 Rabbit mAb (A19585) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] p53 Rabbit mAb (A19585) }

Immunohistochemistry - [KO Validated] p53 Rabbit mAb (A19585)

Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] p53 Rabbit mAb (A19585) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] p53 Rabbit mAb (A19585) }

Immunohistochemistry - [KO Validated] p53 Rabbit mAb (A19585)

Immunohistochemistry of paraffin-embedded Human hepatocholangiocarcinoma using [KO Validated] p53 Rabbit mAb (A19585) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] p53 Rabbit mAb (A19585) }

Immunohistochemistry - [KO Validated] p53 Rabbit mAb (A19585)

Immunohistochemistry of paraffin-embedded Human liver (negative control sample) using [KO Validated] p53 Rabbit mAb (A19585) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunoprecipitation - [KO Validated] p53 Rabbit mAb (A19585) }

Immunoprecipitation - [KO Validated] p53 Rabbit mAb (A19585)

Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug p53 antibody (A19585). Western blot was performed from the immunoprecipitate using p53 antibody (A19585) at a dilition of 1:1000.

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