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[KO Validated] c-Myc Rabbit mAb (A19032)

Reviews (2)Publications (3) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse

ABclonal: - [KO Validated] c-Myc Rabbit mAb (A19032)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 105 K562 cells with 2 ug c-Myc Rabbit mAb,along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of c-Myc in representative gene loci (NPM1), as shown in figure.

ABclonal:Western blot - [KO Validated] c-Myc Rabbit mAb (A19032)

Western blot analysis of extracts of various cell lines, using c-Myc antibody (A19032) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] c-Myc Rabbit mAb (A19032)

Western blot analysis of extracts of Mouse ovary, using c-Myc antibody (A19032) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Western blot - [KO Validated] c-Myc Rabbit mAb (A19032)

Western blot analysis of extracts from wild type (WT) and c-Myc knockout (KO) HCT116 cells, using c-Myc antibody (A19032) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - [KO Validated] c-Myc Rabbit mAb (A19032)

Immunofluorescence analysis of HeLa cells using c-Myc Rabbit mAb (A19032) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - [KO Validated] c-Myc Rabbit mAb (A19032)

Chromatin immunoprecipitation analysis of extracts of K-562 cells, using c-Myc antibody (A19032) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Review
ABclonal: review for [KO Validated] c-Myc Rabbit mAb(A19032)
Review
ABclonal: review for [KO Validated] c-Myc Rabbit mAb(A19032)

Overview

Product name[KO Validated] c-Myc Rabbit mAb
Catalog No.A19032
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008]
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human c-Myc (P01106).
SequenceMPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLE
Gene ID4609
Swiss protP01106
SynonymsMRTL; MYCC; bHLHe39; c-Myc; MYC
Calculated MW57kDa
Observed MW50-60kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
ChIP 1:50 - 1:200
CUT&Tag 105 cells /2 μg
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesJurkat, MCF7, Mouse ovary
Cellular locationNucleus, nucleolus, nucleoplasm
Customer validation

(293T MOLT4 IGROV-1, 293T,JURKAT,HL-60,FUOV-1,A549,MCF-7,T47D,MOLT-4,IGROV-1)

WB(Homo sapiens, Mus musculus)

ABclonal: - [KO Validated] c-Myc Rabbit mAb (A19032)}

- [KO Validated] c-Myc Rabbit mAb (A19032)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 105 K562 cells with 2 ug c-Myc Rabbit mAb,along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of c-Myc in representative gene loci (NPM1), as shown in figure.
ABclonal:Western blot - [KO Validated] c-Myc Rabbit mAb (A19032)}

Western blot - [KO Validated] c-Myc Rabbit mAb (A19032)

Western blot analysis of extracts of various cell lines, using c-Myc antibody (A19032) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] c-Myc Rabbit mAb (A19032)}

Western blot - [KO Validated] c-Myc Rabbit mAb (A19032)

Western blot analysis of extracts of Mouse ovary, using c-Myc antibody (A19032) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
ABclonal:Western blot - [KO Validated] c-Myc Rabbit mAb (A19032)}

Western blot - [KO Validated] c-Myc Rabbit mAb (A19032)

Western blot analysis of extracts from wild type (WT) and c-Myc knockout (KO) HCT116 cells, using c-Myc antibody (A19032) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - [KO Validated] c-Myc Rabbit mAb (A19032)}

Immunofluorescence - [KO Validated] c-Myc Rabbit mAb (A19032)

Immunofluorescence analysis of HeLa cells using c-Myc Rabbit mAb (A19032) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - [KO Validated] c-Myc Rabbit mAb (A19032)}

Chromatin Immunoprecipitation - [KO Validated] c-Myc Rabbit mAb (A19032)

Chromatin immunoprecipitation analysis of extracts of K-562 cells, using c-Myc antibody (A19032) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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