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[KO Validated] c-Myc Rabbit mAb (A19032)

Publications (2) Datasheet

ABclonal:Western blot - [KO Validated] c-Myc Rabbit mAb (A19032)

Western blot analysis of extracts of various cell lines, using c-Myc antibody (A19032) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] c-Myc Rabbit mAb (A19032)

Western blot analysis of extracts of Mouse ovary, using c-Myc antibody (A19032) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Western blot - [KO Validated] c-Myc Rabbit mAb (A19032)

Western blot analysis of extracts from normal (control) and c-Myc knockout (KO) HCT116 cells, using c-Myc antibody (A19032) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - [KO Validated] c-Myc Rabbit mAb (A19032)

Immunofluorescence analysis of HeLa cells using c-Myc Rabbit mAb (A19032) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - [KO Validated] c-Myc Rabbit mAb (A19032)

Chromatin immunoprecipitation analysis of extracts of K-562 cells, using c-Myc antibody (A19032) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product name[KO Validated] c-Myc Rabbit mAb
Catalog No.A19032
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008]
ImmunogenA synthesized peptide derived from human c-Myc
SequenceEmail for sequence
Gene ID4609
Swiss protP01106
SynonymsMRTL; MYCC; bHLHe39; c-Myc; MYC
Calculated MW57kDa
Observed MW50-60kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
ChIP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesJurkat, MCF7, Mouse ovary
Cellular locationNucleus, nucleolus, nucleoplasm
Customer validation

WB(Homo sapiens, Mus musculus)

ABclonal:Western blot - [KO Validated] c-Myc Rabbit mAb (A19032) }

Western blot - [KO Validated] c-Myc Rabbit mAb (A19032)

Western blot analysis of extracts of various cell lines, using c-Myc antibody (A19032) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] c-Myc Rabbit mAb (A19032) }

Western blot - [KO Validated] c-Myc Rabbit mAb (A19032)

Western blot analysis of extracts of Mouse ovary, using c-Myc antibody (A19032) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
ABclonal:Western blot - [KO Validated] c-Myc Rabbit mAb (A19032) }

Western blot - [KO Validated] c-Myc Rabbit mAb (A19032)

Western blot analysis of extracts from normal (control) and c-Myc knockout (KO) HCT116 cells, using c-Myc antibody (A19032) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - [KO Validated] c-Myc Rabbit mAb (A19032) }

Immunofluorescence - [KO Validated] c-Myc Rabbit mAb (A19032)

Immunofluorescence analysis of HeLa cells using c-Myc Rabbit mAb (A19032) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - [KO Validated] c-Myc Rabbit mAb (A19032) }

Chromatin Immunoprecipitation - [KO Validated] c-Myc Rabbit mAb (A19032)

Chromatin immunoprecipitation analysis of extracts of K-562 cells, using c-Myc antibody (A19032) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

* For research use only. Not for therapeutic or diagnostic purposes.

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