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[KO Validated] beta Catenin Rabbit mAb (A19657)

Publications (2) Datasheet

ABclonal:Western blot - [KO Validated] beta Catenin  Rabbit mAb (A19657)
ABclonal:Western blot - [KO Validated] beta Catenin  Rabbit mAb (A19657)
ABclonal:Western blot - [KO Validated] beta Catenin  Rabbit mAb (A19657)
ABclonal:Immunohistochemistry - [KO Validated] beta Catenin  Rabbit mAb (A19657)
ABclonal:Immunohistochemistry - [KO Validated] beta Catenin  Rabbit mAb (A19657)
ABclonal:Immunofluorescence - [KO Validated] beta Catenin  Rabbit mAb (A19657)

Overview

Product name[KO Validated] beta Catenin Rabbit mAb
Catalog No.A19657
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
ImmunogenA synthesized peptide derived from human beta Catenin.
SequenceEmail for sequence
Gene ID1499
Swiss protP35222
SynonymsCTNNB; MRD19; armadillo; beta Catenin; CTNNB1
Calculated MW92kDa
Observed MW92kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Mouse brain, 293T
Cellular location
Customer validation

WB(Homo sapiens, Rattus norvegicus)

IHC(Rattus norvegicus)

ABclonal:Western blot - [KO Validated] beta Catenin  Rabbit mAb (A19657) }

Western blot - [KO Validated] beta Catenin Rabbit mAb (A19657)

Western blot analysis of extracts of HeLa cells, using beta Catenin antibody (A19657) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] beta Catenin  Rabbit mAb (A19657) }

Western blot - [KO Validated] beta Catenin Rabbit mAb (A19657)

Western blot analysis of extracts of Mouse brain, using beta Catenin antibody (A19657) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] beta Catenin  Rabbit mAb (A19657) }

Western blot - [KO Validated] beta Catenin Rabbit mAb (A19657)

Western blot analysis of extracts from normal (control) and beta Catenin knockout (KO) 293T cells, using beta Catenin antibody (A19657) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] beta Catenin  Rabbit mAb (A19657) }

Immunohistochemistry - [KO Validated] beta Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded Rat pancreas using beta Catenin antibody (A19657) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] beta Catenin  Rabbit mAb (A19657) }

Immunohistochemistry - [KO Validated] beta Catenin Rabbit mAb (A19657)

Immunohistochemistry of paraffin-embedded Human colon carcinoma using beta Catenin antibody (A19657) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] beta Catenin  Rabbit mAb (A19657) }

Immunofluorescence - [KO Validated] beta Catenin Rabbit mAb (A19657)

Immunofluorescence analysis of C6 cells using beta Catenin antibody (A19657) at dilution of 1:100. Blue: DAPI for nuclear staining.

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