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[KO Validated] beta 2 Microglobulin Rabbit pAb (A12404)

Datasheet

ABclonal:Western blot - [KO Validated] beta 2 Microglobulin Polyclonal Antibody (A12404)
ABclonal:Western blot - [KO Validated] beta 2 Microglobulin Polyclonal Antibody (A12404)
ABclonal:Immunohistochemistry - [KO Validated] beta 2 Microglobulin Polyclonal Antibody (A12404)
ABclonal:Immunohistochemistry - [KO Validated] beta 2 Microglobulin Polyclonal Antibody (A12404)

Overview

Product name[KO Validated] beta 2 Microglobulin Rabbit pAb
Catalog No.A12404
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human beta 2 Microglobulin (NP_004039.1).
SequenceIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
Gene ID567
Swiss protP61769
SynonymsB2M; IMD43
Calculated MW13kDa
Observed MW12kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesJurkat, COS7, HeLa, K-562, Mouse liver, Mouse spleen
Cellular locationSecreted
ABclonal:Western blot - [KO Validated] beta 2 Microglobulin Polyclonal Antibody (A12404) }

Western blot - [KO Validated] beta 2 Microglobulin Polyclonal Antibody (A12404)

Western blot analysis of extracts of various cell lines, using beta 2 Microglobulin antibody (A12404) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
ABclonal:Western blot - [KO Validated] beta 2 Microglobulin Polyclonal Antibody (A12404) }

Western blot - [KO Validated] beta 2 Microglobulin Polyclonal Antibody (A12404)

Western blot analysis of extracts from normal (control) and beta 2 Microglobulin knockout (KO) 293T cells, using beta 2 Microglobulin antibody (A12404) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] beta 2 Microglobulin Polyclonal Antibody (A12404) }

Immunohistochemistry - [KO Validated] beta 2 Microglobulin Polyclonal Antibody (A12404)

Immunohistochemistry of paraffin-embedded rat brain using beta 2 Microglobulin antibody (A12404) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] beta 2 Microglobulin Polyclonal Antibody (A12404) }

Immunohistochemistry - [KO Validated] beta 2 Microglobulin Polyclonal Antibody (A12404)

Immunohistochemistry of paraffin-embedded human prostate using beta 2 Microglobulin antibody (A12404) at dilution of 1:100 (40x lens).

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