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[KO Validated] active + pro Caspase-3 Rabbit mAb (A19654)

Publications (29) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654)

Western blot analysis of extracts of various cell lines, using active + pro Caspase-3 antibody (A19654) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654)

Western blot analysis of extracts of various cell lines, using active + pro Caspase-3 antibody (A19654) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Western blot - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654)

Western blot analysis of extracts from wild type (WT) and active + pro Caspase 3 knockout (KO) 293T cells, using active+pro Caspase-3 antibody (A19654) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654)

Immunofluorescence analysis of HeLa Treated with Etoposide, HeLa cells using [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] active + pro Caspase-3 Rabbit mAb
Catalog No.A19654
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 29-175 of human Caspase-3 (P42574).
SequenceSGISLDNSYKMDYPEMGLCIIINNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDCGIETD
Gene ID836
Swiss protP42574
SynonymsCPP32; CPP32B; SCA-1; Active Caspase 3; CASP3; active Caspase-3; Caspase 3; Caspase-3 p12; caspase-3
Calculated MW17kDa/32kDa
Observed MW17KDa/32kDa/35KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesJurkat, Mouse lung, Mouse liver, NIH/3T3, Rat spleen, Rat liver, 293T, L929
Cellular locationCytoplasm
Customer validation

WB(Mus musculus, Homo sapiens, Sanghuangporus vaninii, Giardia duodenalis, Rattus norvegicus, Apocynum venetum leaf)

ABclonal:Western blot - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654)}

Western blot - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654)

Western blot analysis of extracts of various cell lines, using active + pro Caspase-3 antibody (A19654) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654)}

Western blot - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654)

Western blot analysis of extracts of various cell lines, using active + pro Caspase-3 antibody (A19654) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Western blot - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654)}

Western blot - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654)

Western blot analysis of extracts from wild type (WT) and active + pro Caspase 3 knockout (KO) 293T cells, using active+pro Caspase-3 antibody (A19654) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654)}

Immunofluorescence - [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654)

Immunofluorescence analysis of HeLa Treated with Etoposide, HeLa cells using [KO Validated] active + pro Caspase-3 Rabbit mAb (A19654) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

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