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[KO Validated] ZMYND8 Rabbit pAb (A8737)

Datasheet

ABclonal:Western blot - [KO Validated] ZMYND8 Polyclonal Antibody (A8737)
ABclonal:Western blot - [KO Validated] ZMYND8 Polyclonal Antibody (A8737)

Overview

Product name[KO Validated] ZMYND8 Rabbit pAb
Catalog No.A8737
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 959-1188 of human ZMYND8 (NP_898868.1).
SequenceLELTMAEMRQSLEQERDRLIAEVKKQLELEKQQAVDETKKKQWCANCKKEAIFYCCWNTSYCDYPCQQAHWPEHMKSCTQSATAPQQEADAEVNTETLNKSSQGSSSSTQSAPSETASASKEKETSAEKSKESGSTLDLSGSRETPSSILLGSNQGSVSKRCDKQPAYAPTTTDHQPHPNYPAQKYHSRSNKSSWSSSDEKRGSTRSDHNTSTSTKSLLPKESRLDTFWD
Gene ID23613
Swiss protQ9ULU4
SynonymsZMYND8; PRKCBP1; PRO2893; RACK7
Calculated MW66kDa/82kDa/109-138kDa
Observed MW165kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:1000 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesU-87 MG, LO2, MCF-7, A375
Cellular location
ABclonal:Western blot - [KO Validated] ZMYND8 Polyclonal Antibody (A8737) }

Western blot - [KO Validated] ZMYND8 Polyclonal Antibody (A8737)

Western blot analysis of extracts of various cell lines, using ZMYND8 antibody (A8737) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] ZMYND8 Polyclonal Antibody (A8737) }

Western blot - [KO Validated] ZMYND8 Polyclonal Antibody (A8737)

Western blot analysis of extracts from normal (control) and ZMYND8 knockout (KO) HeLa cells, using ZMYND8 antibody (A8737) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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