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[KO Validated] YBX1 Rabbit pAb (A7704)

Datasheet

ABclonal:Western blot - [KO Validated] YBX1 Rabbit pAb (A7704)
ABclonal:Western blot - [KO Validated] YBX1 Rabbit pAb (A7704)
ABclonal:Immunohistochemistry - [KO Validated] YBX1 Rabbit pAb (A7704)
ABclonal:Immunohistochemistry - [KO Validated] YBX1 Rabbit pAb (A7704)
ABclonal:Immunohistochemistry - [KO Validated] YBX1 Rabbit pAb (A7704)
ABclonal:Immunofluorescence - [KO Validated] YBX1 Rabbit pAb (A7704)
ABclonal:Immunofluorescence - [KO Validated] YBX1 Rabbit pAb (A7704)
ABclonal:Immunofluorescence - [KO Validated] YBX1 Rabbit pAb (A7704)

Overview

Product name[KO Validated] YBX1 Rabbit pAb
Catalog No.A7704
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human YBX1 (NP_004550.2).
SequenceLRSVGDGETVEFDVVEGEKGAEAANVTGPGGVPVQGSKYAADRNHYRRYPRRRGPPRNYQQNYQNSESGEKNEGSESAPEGQAQQRRPYRRRRFPPYYMRR
Gene ID4904
Swiss protP67809
SynonymsYBX1; BP-8; CBF-A; CSDA2; CSDB; DBPB; EFI-A; MDR-NF1; NSEP-1; NSEP1; YB-1; YB1
Calculated MW35kDa
Observed MW49kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2
Cellular locationCytoplasm, Cytoplasmic granule, Nucleus, Secreted
ABclonal:Western blot - [KO Validated] YBX1 Rabbit pAb (A7704) }

Western blot - [KO Validated] YBX1 Rabbit pAb (A7704)

Western blot analysis of extracts of HepG2 cells, using YBX1 antibody (A7704) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] YBX1 Rabbit pAb (A7704) }

Western blot - [KO Validated] YBX1 Rabbit pAb (A7704)

Western blot analysis of extracts from normal (control) and YBX1 knockout (KO) HeLa cells, using YBX1 antibody (A7704) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - [KO Validated] YBX1 Rabbit pAb (A7704) }

Immunohistochemistry - [KO Validated] YBX1 Rabbit pAb (A7704)

Immunohistochemistry of paraffin-embedded rat testis using YBX1 antibody (A7704) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] YBX1 Rabbit pAb (A7704) }

Immunohistochemistry - [KO Validated] YBX1 Rabbit pAb (A7704)

Immunohistochemistry of paraffin-embedded mouse testis using YBX1 antibody (A7704) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] YBX1 Rabbit pAb (A7704) }

Immunohistochemistry - [KO Validated] YBX1 Rabbit pAb (A7704)

Immunohistochemistry of paraffin-embedded human colon carcinoma using YBX1 antibody (A7704) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] YBX1 Rabbit pAb (A7704) }

Immunofluorescence - [KO Validated] YBX1 Rabbit pAb (A7704)

Immunofluorescence analysis of mouse colon using YBX1 antibody (A7704) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] YBX1 Rabbit pAb (A7704) }

Immunofluorescence - [KO Validated] YBX1 Rabbit pAb (A7704)

Immunofluorescence analysis of mouse colone artery using YBX1 antibody (A7704) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] YBX1 Rabbit pAb (A7704) }

Immunofluorescence - [KO Validated] YBX1 Rabbit pAb (A7704)

Immunofluorescence analysis of mouse brain using YBX1 antibody (A7704) at dilution of 1:100. Blue: DAPI for nuclear staining.

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