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[KO Validated] YAP1 Polyclonal Antibody (A1002)

Reviews (8)Publications (5) Datasheet

ABclonal:Western blot - [KO Validated] YAP1 Polyclonal Antibody (A1002)
ABclonal:Western blot - [KO Validated] YAP1 Polyclonal Antibody (A1002)
ABclonal:Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (A1002)
ABclonal:Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (A1002)
ABclonal:Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (A1002)
ABclonal:Immunofluorescence - [KO Validated] YAP1 Polyclonal Antibody (A1002)
ABclonal:Immunofluorescence - [KO Validated] YAP1 Polyclonal Antibody (A1002)
ABclonal:Immunofluorescence - [KO Validated] YAP1 Polyclonal Antibody (A1002)
ABclonal:Immunoprecipitation - [KO Validated] YAP1 Polyclonal Antibody (A1002)
Review
ABclonal: review for [KO Validated] YAP1 Polyclonal Antibody(A1002)
Review
ABclonal: review for [KO Validated] YAP1 Polyclonal Antibody(A1002)
Review
ABclonal: review for [KO Validated] YAP1 Polyclonal Antibody(A1002)
Review
ABclonal: review for [KO Validated] YAP1 Polyclonal Antibody(A1002)
Review
ABclonal: review for [KO Validated] YAP1 Polyclonal Antibody(A1002)
Review
ABclonal: review for [KO Validated] YAP1 Polyclonal Antibody(A1002)
Review
ABclonal: review for [KO Validated] YAP1 Polyclonal Antibody(A1002)
Review
ABclonal: review for [KO Validated] YAP1 Polyclonal Antibody(A1002)

Overview

Product name[KO Validated] YAP1 Polyclonal Antibody
Catalog No.A1002
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1).
SequencePTAQHLRQSSFEIPDDVPLPAGWEMAKTSSGQRYFLNHIDQTTTWQDPRKAMLSQMNVTAPTSPPVQQNMMNSASGPLPDGWEQAMTQDGEIYYINHKNKTTSWLDPRLDPRFAMNQRISQSAPVKQPPPLAPQSPQGGVMGGSNSNQQQQMRLQQLQMEKERLRLKQQELLRQAMRNINPSTANSPKCQELALRSQLPTLEQDGGTQNPVSSPGMSQELRTMTTNSSDPFLNSGTYHSRDESTDSGLSMSSYSVPRTPDDFLNSVDEMDTGDTINQSTLPSQQNRFPDYLEAIPGTNVDLGTLEGDGMNIEGEELMPSLQEALSSDILNDMESVLAATKLDKESFLTWL
Gene ID10413
Swiss protP46937
SynonymsYAP1; COB1; YAP; YAP2; YAP65; YKI; Yes associated protein 1
Calculated MW36kDa/48kDa/49kDa/50kDa/52kDa/53kDa/54kDa
Observed MW72kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, LO2, BT-474, A549
Cellular locationCytoplasm, Nucleus
Customer validation

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WB(Human)

IP(Human)

IF(Human)

IF(Human)

ChIP(Human)

IHC(Human)

ABclonal:Western blot - [KO Validated] YAP1 Polyclonal Antibody (A1002) }

Western blot - [KO Validated] YAP1 Polyclonal Antibody (A1002)

Western blot analysis of extracts of various cell lines, using YAP1 antibody (A1002) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] YAP1 Polyclonal Antibody (A1002) }

Western blot - [KO Validated] YAP1 Polyclonal Antibody (A1002)

Western blot analysis of extracts from normal (control) and YAP1 knockout (KO) HeLa cells, using YAP1 antibody (A1002) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (A1002) }

Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (A1002)

Immunohistochemistry of paraffin-embedded human placenta using YAP1 antibody (A1002) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (A1002) }

Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (A1002)

Immunohistochemistry of paraffin-embedded rat kidney using YAP1 antibody (A1002) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (A1002) }

Immunohistochemistry - [KO Validated] YAP1 Polyclonal Antibody (A1002)

Immunohistochemistry of paraffin-embedded mouse heart using YAP1 antibody (A1002) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] YAP1 Polyclonal Antibody (A1002) }

Immunofluorescence - [KO Validated] YAP1 Polyclonal Antibody (A1002)

Immunofluorescence analysis of HeLa cells using YAP1 antibody (A1002) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] YAP1 Polyclonal Antibody (A1002) }

Immunofluorescence - [KO Validated] YAP1 Polyclonal Antibody (A1002)

Immunofluorescence analysis of NIH/3T3 cells using YAP1 antibody (A1002) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] YAP1 Polyclonal Antibody (A1002) }

Immunofluorescence - [KO Validated] YAP1 Polyclonal Antibody (A1002)

Immunofluorescence analysis of PC12 cells using YAP1 antibody (A1002) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] YAP1 Polyclonal Antibody (A1002) }

Immunoprecipitation - [KO Validated] YAP1 Polyclonal Antibody (A1002)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug YAP1 antibody (A1002). Western blot was performed from the immunoprecipitate using YAP1 antibody (A1002) at a dilition of 1:1000.

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