Products > Catalog Antibodies > Polyclonal Antibodies

[KO Validated] XPA Rabbit pAb (A1626)

Datasheet

ABclonal:Western blot - [KO Validated] XPA Polyclonal Antibody (A1626)
ABclonal:Western blot - [KO Validated] XPA Polyclonal Antibody (A1626)
ABclonal:Immunofluorescence - [KO Validated] XPA Polyclonal Antibody (A1626)
ABclonal:Immunofluorescence - [KO Validated] XPA Polyclonal Antibody (A1626)

Overview

Product name[KO Validated] XPA Rabbit pAb
Catalog No.A1626
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human XPA (NP_000371.1).
SequenceMAAADGALPEAAALEQPAELPASVRASIERKRQRALMLRQARLAARPYSATAAAATGGMANVKAAPKIIDTGGGFILEEEEEEEQKIGKVVHQPGPVMEFDYVICEECGKEFMDSYLMNHFDLPTCDNCRDADDKHKLITKTEAKQEYLLKDCDLEKREPPLKFIVKKNPHHSQWGDMKLYLKLQIVKRSLEVWGSQEALEEAKEVRQENREKMKQKKFDKKVKELRRAVRSSVWKRETIVHQHEYGPEENLEDDMYRKTCTMCGHELTYEKM
Gene ID7507
Swiss protP23025
SynonymsXPA; XP1; XPAC
Calculated MW31kDa
Observed MW37kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa, Jurkat, K-562, Mouse liver, Mouse kidney
Cellular locationNucleus
ABclonal:Western blot - [KO Validated] XPA Polyclonal Antibody (A1626) }

Western blot - [KO Validated] XPA Polyclonal Antibody (A1626)

Western blot analysis of extracts of various cell lines, using XPA antibody (A1626) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] XPA Polyclonal Antibody (A1626) }

Western blot - [KO Validated] XPA Polyclonal Antibody (A1626)

Western blot analysis of extracts from normal (control) and XPA knockout (KO) HeLa cells, using XPA antibody (A1626) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Immunofluorescence - [KO Validated] XPA Polyclonal Antibody (A1626) }

Immunofluorescence - [KO Validated] XPA Polyclonal Antibody (A1626)

Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using XPA antibody (A1626). Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
ABclonal:Immunofluorescence - [KO Validated] XPA Polyclonal Antibody (A1626) }

Immunofluorescence - [KO Validated] XPA Polyclonal Antibody (A1626)

Immunofluorescence analysis of A549 cells using XPA antibody (A1626).

Inquire About This Product

Submit your question about A1626 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
Contact us to order