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[KO Validated] XIAP Rabbit pAb (A6869)

Publications (3) Datasheet

ABclonal:Western blot - [KO Validated] XIAP Polyclonal Antibody (A6869)
ABclonal:Western blot - [KO Validated] XIAP Polyclonal Antibody (A6869)
ABclonal:Immunohistochemistry - [KO Validated] XIAP Polyclonal Antibody (A6869)
ABclonal:Immunohistochemistry - [KO Validated] XIAP Polyclonal Antibody (A6869)
ABclonal:Immunofluorescence - [KO Validated] XIAP Polyclonal Antibody (A6869)

Overview

Product name[KO Validated] XIAP Rabbit pAb
Catalog No.A6869
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human XIAP (NP_001158.2).
SequenceMTFNSFEGSKTCVPADINKEEEFVEEFNRLKTFANFPSGSPVSASTLARAGFLYTGEGDTVRCFSCHAAVDRWQYGDSAVGRHRKVSPNCRFINGFYLENSATQSTNSGIQNGQYKVENYLGSRDHFALDRPSETHADYLLRTGQVVDISDTIYPRNPAMYSEEARLKSFQNWPDYAHLTPRELASAGLYYTGIGDQVQC
Gene ID331
Swiss protP98170
SynonymsXIAP; API3; BIRC4; IAP-3; ILP1; MIHA; XLP2; hIAP-3; hIAP3
Calculated MW56kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF-7, HeLa, SW620, HepG2, Jurkat, Mouse liver, Rat kidney
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mus musculus, Homo sapiens)

IHC(Mus musculus)

IF(Mus musculus)

ABclonal:Western blot - [KO Validated] XIAP Polyclonal Antibody (A6869) }

Western blot - [KO Validated] XIAP Polyclonal Antibody (A6869)

Western blot analysis of extracts of various cell lines, using XIAP antibody (A6869) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] XIAP Polyclonal Antibody (A6869) }

Western blot - [KO Validated] XIAP Polyclonal Antibody (A6869)

Western blot analysis of extracts from normal (control) and XIAP knockout (KO) HeLa cells, using XIAP antibody (A6869) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - [KO Validated] XIAP Polyclonal Antibody (A6869) }

Immunohistochemistry - [KO Validated] XIAP Polyclonal Antibody (A6869)

Immunohistochemistry of paraffin-embedded human kidney using XIAP antibody (A6869) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] XIAP Polyclonal Antibody (A6869) }

Immunohistochemistry - [KO Validated] XIAP Polyclonal Antibody (A6869)

Immunohistochemistry of paraffin-embedded mouse kidney using XIAP antibody (A6869) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] XIAP Polyclonal Antibody (A6869) }

Immunofluorescence - [KO Validated] XIAP Polyclonal Antibody (A6869)

Immunofluorescence analysis of MCF7 cells using XIAP antibody (A6869).

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