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[KO Validated] Vimentin Rabbit pAb (A2666)

Publications (5) Datasheet

ABclonal:Western blot - [KO Validated] Vimentin Rabbit pAb (A2666)

Western blot analysis of extracts of various cell lines, using Vimentin antibody (A2666) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Western blot - [KO Validated] Vimentin Rabbit pAb (A2666)

Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) HeLa cells, using Vimentin antibody (A2666) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name[KO Validated] Vimentin Rabbit pAb
Catalog No.A2666
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
ImmunogenRecombinant protein of human Vimentin
SequenceEmail for sequence
Gene ID7431
Swiss protP08670
SynonymsCTRCT30; HEL113; Vimentin; VIM; vimentin
Calculated MW53kDa
Observed MW55kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesU-251MG, HeLa, A-549, MCF-7
Cellular locationCytoplasm
Customer validation

WB(Homo sapiens, Mus musculus)

IF(Homo sapiens)

IF(Homo sapiens,Mus musculus)

IHC(Homo sapiens,Mus musculus)

ABclonal:Western blot - [KO Validated] Vimentin Rabbit pAb (A2666) }

Western blot - [KO Validated] Vimentin Rabbit pAb (A2666)

Western blot analysis of extracts of various cell lines, using Vimentin antibody (A2666) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] Vimentin Rabbit pAb (A2666) }

Western blot - [KO Validated] Vimentin Rabbit pAb (A2666)

Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) HeLa cells, using Vimentin antibody (A2666) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

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