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[KO Validated] Vimentin Rabbit mAb (A19607)

Publications (19) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:[KO Validated] Vimentin Rabbit mAb
ABclonal:Western blot - [KO Validated] Vimentin Rabbit mAb (A19607)

Western blot analysis of extracts of various cell lines, using Vimentin antibody (A19607) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] Vimentin Rabbit mAb (A19607)

Western blot analysis of extracts from wild type (WT) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (A19607) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - [KO Validated] Vimentin Rabbit mAb (A19607)

Immunohistochemistry of paraffin-embedded Human liver using Vimentin antibody (A19607) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] Vimentin Rabbit mAb (A19607)

Immunohistochemistry of paraffin-embedded Human colon carcinoma using Vimentin antibody (A19607) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] Vimentin Rabbit mAb (A19607)

Immunohistochemistry of paraffin-embedded Human kidney using Vimentin antibody (A19607) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] Vimentin Rabbit mAb (A19607)

Confocal Immunofluorescence analysis of U2OS cells using KRR1 Rabbit pAb (A4487) at dilution of 1:100 (40x lens)(red). Vimentin (A19607: Vimentin Rabbit mAb) for Cytoskeleton staining(green), DAPI for nuclear staining(blue).

ABclonal:Immunoprecipitation - [KO Validated] Vimentin Rabbit mAb (A19607)

Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Vimentin antibody (A19607). Western blot was performed from the immunoprecipitate using Vimentin antibody (A19607) at a dilition of 1:1000.

Overview

Product name[KO Validated] Vimentin Rabbit mAb
Catalog No.A19607
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 367-466 of human Vimentin (P08670).
SequenceDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE
Gene ID7431
Swiss protP08670
SynonymsCTRCT30; HEL113; Vimentin; VIM; vimentin
Calculated MW54kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293T, Jurkat, HeLa, Mouse testis, C6
Cellular locationCytoplasm
Customer validation

WB(Homo sapiens, Mus musculus)

IF(Mus musculus)

IHC(Homo sapiens)

IF(Mus musculus, Homo sapiens)

IF(Homo sapiens)

ABclonal:Western blot - [KO Validated] Vimentin Rabbit mAb (A19607)}

Western blot - [KO Validated] Vimentin Rabbit mAb (A19607)

Western blot analysis of extracts of various cell lines, using Vimentin antibody (A19607) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] Vimentin Rabbit mAb (A19607)}

Western blot - [KO Validated] Vimentin Rabbit mAb (A19607)

Western blot analysis of extracts from wild type (WT) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (A19607) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] Vimentin Rabbit mAb (A19607)}

Immunohistochemistry - [KO Validated] Vimentin Rabbit mAb (A19607)

Immunohistochemistry of paraffin-embedded Human liver using Vimentin antibody (A19607) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] Vimentin Rabbit mAb (A19607)}

Immunohistochemistry - [KO Validated] Vimentin Rabbit mAb (A19607)

Immunohistochemistry of paraffin-embedded Human colon carcinoma using Vimentin antibody (A19607) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] Vimentin Rabbit mAb (A19607)}

Immunohistochemistry - [KO Validated] Vimentin Rabbit mAb (A19607)

Immunohistochemistry of paraffin-embedded Human kidney using Vimentin antibody (A19607) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - [KO Validated] Vimentin Rabbit mAb (A19607)}

Immunofluorescence - [KO Validated] Vimentin Rabbit mAb (A19607)

Confocal Immunofluorescence analysis of U2OS cells using KRR1 Rabbit pAb (A4487) at dilution of 1:100 (40x lens)(red). Vimentin (A19607: Vimentin Rabbit mAb) for Cytoskeleton staining(green), DAPI for nuclear staining(blue).
ABclonal:Immunoprecipitation - [KO Validated] Vimentin Rabbit mAb (A19607)}

Immunoprecipitation - [KO Validated] Vimentin Rabbit mAb (A19607)

Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Vimentin antibody (A19607). Western blot was performed from the immunoprecipitate using Vimentin antibody (A19607) at a dilition of 1:1000.

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