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[KO Validated] Vimentin Rabbit mAb (A19607)

Tested applications:WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] Vimentin Rabbit mAb (A19607)

Western blot analysis of extracts of various cell lines, using Vimentin antibody (A19607) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of extracts from wild type (WT) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (A19607) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - [KO Validated] Vimentin Rabbit mAb (A19607)

Immunohistochemistry of paraffin-embedded Human liver using Vimentin antibody (A19607) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded Human colon carcinoma using Vimentin antibody (A19607) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded Human kidney using Vimentin antibody (A19607) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] Vimentin Rabbit mAb (A19607)

Confocal Immunofluorescence analysis of U2OS cells using KRR1 Rabbit pAb (A4487) at dilution of 1:100 (40x lens)(red). Vimentin (A19607: Vimentin Rabbit mAb) for Cytoskeleton staining(green), DAPI for nuclear staining(blue).

ABclonal:Immunoprecipitation - [KO Validated] Vimentin Rabbit mAb (A19607)

Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Vimentin antibody (A19607). Western blot was performed from the immunoprecipitate using Vimentin antibody (A19607) at a dilition of 1:1000.

Overview

Product name	[KO Validated] Vimentin Rabbit mAb
Catalog No.	A19607
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 367-466 of human Vimentin (P08670).
Sequence	DEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE
Gene ID	7431
Swiss prot	P08670
Synonyms	CTRCT30; HEL113; Vimentin; VIM; vimentin
Calculated MW	54kDa
Observed MW	54kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	293T, Jurkat, HeLa, Mouse testis, C6
Cellular location	Cytoplasm
Customer validation	WB(Homo sapiens, Mus musculus) IF(Mus musculus) IHC(Homo sapiens) IF(Mus musculus, Homo sapiens) IF(Homo sapiens)

Research Area

[KO Validated] Vimentin Rabbit mAb images

Western blot - [KO Validated] Vimentin Rabbit mAb (A19607)

Immunohistochemistry - [KO Validated] Vimentin Rabbit mAb (A19607)

Immunofluorescence - [KO Validated] Vimentin Rabbit mAb (A19607)

Immunoprecipitation - [KO Validated] Vimentin Rabbit mAb (A19607)

Product Protocols

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References for [KO Validated] Vimentin Rabbit mAb (A19607)

Product:

[KO Validated] Vimentin Rabbit mAb

Journal:

Cell Biochem Funct

Application:

IF:

3.685

Species:

Homo sapiens

PMID:

32881051

Title:

ALDH5A1 acts as a tumour promoter and has a prognostic impact in papillary thyroid carcinoma

References for [KO Validated] Vimentin Rabbit mAb (A19607)

Product:

[KO Validated] Vimentin Rabbit mAb

Journal:

Exp Physiol

Application:

IF:

2.969

Species:

Mus musculus

PMID:

32936966

Title:

IGFBP3 inhibits angiotensin II‐induced aortic smooth muscle cell phenotypic switch and MMPs expression

References for [KO Validated] Vimentin Rabbit mAb (A19607)

Product:

[KO Validated] Vimentin Rabbit mAb

Journal:

Developmental Cell

Application:

IF:

12.27

Species:

Homo sapiens

PMID:

33238152

Title:

A Single-Cell Transcriptomic Atlas of Human Skin Aging

References for [KO Validated] Vimentin Rabbit mAb (A19607)

Product:

[KO Validated] Vimentin Rabbit mAb

Journal:

Onco Targets Ther

Application:

IF:

4.147

Species:

Homo sapiens

PMID:

33658804

Title:

TRIM11 Promotes Proliferation, Migration, Invasion and EMT of Gastric Cancer by Activating β-Catenin Signaling

References for [KO Validated] Vimentin Rabbit mAb (A19607)

Product:

[KO Validated] Vimentin Rabbit mAb

Journal:

Acta Trop

Application:

WB, IF

IF:

3.11

Species:

Mus musculus

PMID:

34389327

Title:

Microcyst fluid promotes the migration and invasion of fibroblasts in the adventitial layer of alveolar echinococcosis

References for [KO Validated] Vimentin Rabbit mAb (A19607)

Product:

[KO Validated] Vimentin Rabbit mAb

Journal:

Cancer Gene Ther

Application:

IHC

IF:

5.987

Species:

Homo sapiens

PMID:

33531647

Title:

LncRNA HLA-F-AS1 promotes colorectal cancer metastasis by inducing PFN1 in colorectal cancer-derived extracellular vesicles and mediating macrophage polarization

References for [KO Validated] Vimentin Rabbit mAb (A19607)

Product:

[KO Validated] Vimentin Rabbit mAb

Journal:

Exp Anim

Application:

IF:

2.31

Species:

Mus musculus

PMID:

33658464

Title:

MiR-133a-3p inhibits scar formation in scalded mice and suppresses the proliferation and migration of scar derived-fibroblasts by targeting CTGF

References for [KO Validated] Vimentin Rabbit mAb (A19607)

Product:

[KO Validated] Vimentin Rabbit mAb

Journal:

Cancer Med

Application:

WB, IF

IF:

Species:

Homo sapiens

PMID:

33838016

Title:

miR-140-3p inhibits colorectal cancer progression and its liver metastasis by targeting BCL9 and BCL2

References for [KO Validated] Vimentin Rabbit mAb (A19607)

Product:

[KO Validated] Vimentin Rabbit mAb

Journal:

Cancer Med

Application:

IF:

4.452

Species:

Mus musculus

PMID:

34152094

Title:

HHLA2 deficiency inhibits non‐small cell lung cancer progression and THP‐1 macrophage M2 polarization

References for [KO Validated] Vimentin Rabbit mAb (A19607)

Product:

[KO Validated] Vimentin Rabbit mAb

Journal:

Acta Histochem

Application:

IF:

2.479

Species:

Mus musculus

PMID:

34217047

Title:

Calycosin attenuates pulmonary fibrosis by the epithelial-mesenchymal transition repression upon inhibiting the AKT/GSK3β/β-catenin signaling pathway

References for [KO Validated] Vimentin Rabbit mAb (A19607)

Product:

[KO Validated] Vimentin Rabbit mAb

Journal:

Int J Biochem Cell Biol

Application:

IF:

5.085

Species:

Mus musculus

PMID:

34217812

Title:

KIF18B as a regulator in tumor microenvironment accelerates tumor progression and triggers poor outcome in hepatocellular carcinoma

References for [KO Validated] Vimentin Rabbit mAb (A19607)

Product:

[KO Validated] Vimentin Rabbit mAb

Journal:

Gland Surg

Application:

IF:

2.953

Species:

Homo sapiens

PMID:

34268078

Title:

KIF23 promotes triple negative breast cancer through activating epithelial-mesenchymal transition

References for [KO Validated] Vimentin Rabbit mAb (A19607)

Product:

[KO Validated] Vimentin Rabbit mAb

Journal:

Lab Invest

Application:

IF:

5.662

Species:

Homo sapiens

PMID:

34916611

Title:

LncRNA GAS5 regulates migration and epithelial-to-mesenchymal transition in lens epithelial cells via the miR-204-3p/TGFBR1 axis

References for [KO Validated] Vimentin Rabbit mAb (A19607)

Product:

[KO Validated] Vimentin Rabbit mAb

Journal:

Frontiers in aging neuroscience

Application:

IF:

5.75

Species:

Mus musculus

PMID:

35493946

Title:

Transcranial Direct Current Stimulation Alleviates Neurovascular Unit Dysfunction in Mice With Preclinical Alzheimer's Disease

References for [KO Validated] Vimentin Rabbit mAb (A19607)

Product:

[KO Validated] Vimentin Rabbit mAb

Journal:

Biochemical and biophysical research communications

Application:

IF:

3.57

Species:

Homo sapiens

PMID:

27693697

Title:

Let-7d miRNA prevents TGF-β1-induced EMT and renal fibrogenesis through regulation of HMGA2 expression

References for [KO Validated] Vimentin Rabbit mAb (A19607)

Product:

[KO Validated] Vimentin Rabbit mAb

Journal:

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences

Application:

IF:

4.38

Species:

Homo sapiens

PMID:

31518680

Title:

I-8, a novel inhibitor of mutant IDH1, inhibits cancer progression in vitro and in vivo

References for [KO Validated] Vimentin Rabbit mAb (A19607)

Product:

[KO Validated] Vimentin Rabbit mAb

Journal:

Cell death discovery

Application:

IF:

5.24

Species:

Homo sapiens

PMID:

35418193

Title:

Long noncoding RNA LINC00857 promotes pancreatic cancer proliferation and metastasis by regulating the miR-130b/RHOA axis

References for [KO Validated] Vimentin Rabbit mAb (A19607)

Product:

[KO Validated] Vimentin Rabbit mAb

Journal:

BMC oral health

Application:

IF:

2.75

Species:

Homo sapiens

PMID:

35413908

Title:

Different expression patterns of inflammatory cytokines induced by lipopolysaccharides from Escherichia coli or Porphyromonas gingivalis in human dental pulp stem cells

References for [KO Validated] Vimentin Rabbit mAb (A19607)

Product:

[KO Validated] Vimentin Rabbit mAb

Journal:

Frontiers in bioscience (Landmark edition)

Application:

IF:

Species:

Homo sapiens

PMID:

34856762

Title: