Products > Catalog Antibodies > Polyclonal Antibodies

[KO Validated] Vimentin Rabbit pAb (A11952)

Review (1)Publications (5) Datasheet

ABclonal:Western blot - [KO Validated] Vimentin Polyclonal Antibody (A11952)
ABclonal:Western blot - [KO Validated] Vimentin Polyclonal Antibody (A11952)
ABclonal:Immunohistochemistry - [KO Validated] Vimentin Polyclonal Antibody (A11952)
ABclonal:Immunohistochemistry - [KO Validated] Vimentin Polyclonal Antibody (A11952)
ABclonal:Immunofluorescence - [KO Validated] Vimentin Polyclonal Antibody (A11952)
Review
ABclonal: review for [KO Validated] Vimentin Rabbit pAb(A11952)

Overview

Product name[KO Validated] Vimentin Rabbit pAb
Catalog No.A11952
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human Vimentin (NP_003371.2).
SequenceMSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRT
Gene ID7431
Swiss protP08670
SynonymsCTRCT30; HEL113; Vimentin; VIM; vimentin
Calculated MW53kDa
Observed MW57kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat, HeLa
Cellular locationCytoplasm
Customer validation

(Human glioma cells)

WB(Homo sapiens)

IF(Homo sapiens)

IF(Homo sapiens)

Co-IP(Homo sapiens)

IHC(Homo sapiens)

ABclonal:Western blot - [KO Validated] Vimentin Polyclonal Antibody (A11952) }

Western blot - [KO Validated] Vimentin Polyclonal Antibody (A11952)

Western blot analysis of extracts of various cell lines, using Vimentin antibody (A11952) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] Vimentin Polyclonal Antibody (A11952) }

Western blot - [KO Validated] Vimentin Polyclonal Antibody (A11952)

Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (A11952) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] Vimentin Polyclonal Antibody (A11952) }

Immunohistochemistry - [KO Validated] Vimentin Polyclonal Antibody (A11952)

Immunohistochemistry of paraffin-embedded human tonsil using Vimentin antibody (A11952) at dilution of 1:150 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] Vimentin Polyclonal Antibody (A11952) }

Immunohistochemistry - [KO Validated] Vimentin Polyclonal Antibody (A11952)

Immunohistochemistry of paraffin-embedded human stomach using Vimentin antibody (A11952) at dilution of 1:150 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] Vimentin Polyclonal Antibody (A11952) }

Immunofluorescence - [KO Validated] Vimentin Polyclonal Antibody (A11952)

Immunofluorescence analysis of L929 cells using [KO] Vimentin antibody (A11952) at dilution of 1:100. Blue: DAPI for nuclear staining.

Submit an review for A11952

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A11952 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A11952? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
Contact us to order