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[KO Validated] UBE2O Rabbit pAb (A10036)

Datasheet

ABclonal:Western blot - [KO Validated] UBE2O Rabbit pAb (A10036)
ABclonal:Western blot - [KO Validated] UBE2O Rabbit pAb (A10036)
ABclonal:Immunofluorescence - [KO Validated] UBE2O Rabbit pAb (A10036)
ABclonal:Immunoprecipitation - [KO Validated] UBE2O Rabbit pAb (A10036)

Overview

Product name[KO Validated] UBE2O Rabbit pAb
Catalog No.A10036
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3).
SequenceLVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLEKAQALPNGVPKASSSPEPPAVAELSDSGQQEPEDGGPAPGEASQGSDSEGGAQGLASASRDHTDQTSETAPDASVPPSVKPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPECTEDK
Gene ID63893
Swiss protQ9C0C9
SynonymsUBE2O; E2-230K
Calculated MW141kDa
Observed MW200kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:1000 - 1:4000
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesA-431, HepG2, Jurkat, Mouse heart, Mouse brain
Cellular locationCytoplasm, Nucleus
ABclonal:Western blot - [KO Validated] UBE2O Rabbit pAb (A10036) }

Western blot - [KO Validated] UBE2O Rabbit pAb (A10036)

Western blot analysis of extracts of various cell lines, using UBE2O antibody (A10036) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] UBE2O Rabbit pAb (A10036) }

Western blot - [KO Validated] UBE2O Rabbit pAb (A10036)

Western blot analysis of extracts from normal (control) and UBE2O knockout (KO) HeLa cells, using UBE2O antibody (A10036) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - [KO Validated] UBE2O Rabbit pAb (A10036) }

Immunofluorescence - [KO Validated] UBE2O Rabbit pAb (A10036)

Immunofluorescence analysis of HeLa cells using UBE2O Polyclonal Antibody (A10036) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] UBE2O Rabbit pAb (A10036) }

Immunoprecipitation - [KO Validated] UBE2O Rabbit pAb (A10036)

Immunoprecipitation analysis of 200ug extracts of A-431 cells using 3ug UBE2O antibody (A10036). Western blot was performed from the immunoprecipitate using UBE2O antibody (A10036) at a dilition of 1:1000.

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