Western blot analysis of extracts of various cell lines, using TTC11/FIS1 antibody (A19666) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts from normal (control) and TTC11/FIS1 knockout (KO) HeLa cells, using TTC11/FIS1 antibody (A19666) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat brain using TTC11/FIS1 antibody (A19666) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using TTC11/FIS1 antibody (A19666) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using TTC11/FIS1 antibody (A19666) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using TTC11/FIS1 antibody (A19666). Blue: DAPI for nuclear staining.
| Product name | [KO Validated] TTC11/FIS1 Rabbit mAb |
|---|---|
| Catalog No. | A19666 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthesized peptide derived from human TTC11/FIS1. |
|---|---|
| Sequence | Email for sequence |
| Gene ID | 51024 |
| Swiss prot | Q9Y3D6 |
| Synonyms | CGI-135; TTC11; FIS1; TTC11 / FIS1; fission |
| Calculated MW | 15kDa |
| Observed MW | 15KDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation |
| Positive samples | HeLa, HepG2, 293T, NIH/3T3, Mouse liver, Rat liver |
| Cellular location | Mitochondrion outer membrane, Peroxisome membrane, Single-pass membrane protein |
| Customer validation | WB(Mus musculus) |
* For research use only. Not for therapeutic or diagnostic purposes.
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![ABclonal:Western blot - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)](/Uploads/Catalog/A19666/A19666_1.jpg?t=1642561950 "ABclonal:Western blot - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)")
![ABclonal:Western blot - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)](/Uploads/Catalog/A19666/A19666_2.jpg?t=1642561955 "ABclonal:Western blot - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)")
![ABclonal:Immunohistochemistry - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)](/Uploads/Catalog/A19666/A19666_3.jpg?t=1642561968 "ABclonal:Immunohistochemistry - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)")
![ABclonal:Immunohistochemistry - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)](/Uploads/Catalog/A19666/A19666_4.jpg?t=1642561977 "ABclonal:Immunohistochemistry - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)")
![ABclonal:Immunohistochemistry - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)](/Uploads/Catalog/A19666/A19666_5.jpg?t=1642561985 "ABclonal:Immunohistochemistry - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)")
![ABclonal:Immunofluorescence - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)](/Uploads/Catalog/A19666/A19666_6.jpg?t=1642561988 "ABclonal:Immunofluorescence - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)")
![ABclonal:Immunoprecipitation - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)](/Uploads/Catalog/A19666/A19666_7.jpg?t=1642561990 "ABclonal:Immunoprecipitation - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)")
