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[KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Publications (2) Datasheet

ABclonal:Western blot - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Western blot analysis of extracts of various cell lines, using TTC11/FIS1 antibody (A19666) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Western blot analysis of extracts from normal (control) and TTC11/FIS1 knockout (KO) HeLa cells, using TTC11/FIS1 antibody (A19666) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Immunohistochemistry of paraffin-embedded rat brain using TTC11/FIS1 antibody (A19666) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Immunohistochemistry of paraffin-embedded human lung cancer using TTC11/FIS1 antibody (A19666) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Immunohistochemistry of paraffin-embedded mouse kidney using TTC11/FIS1 antibody (A19666) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Immunofluorescence analysis of NIH-3T3 cells using TTC11/FIS1 antibody (A19666). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug TTC11/FIS1 antibody (A19666). Western blot was performed from the immunoprecipitate using TTC11/FIS1 antibody (A19666) at a dilition of 1:1000.

Overview

Product name[KO Validated] TTC11/FIS1 Rabbit mAb
Catalog No.A19666
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM, Mar 2008]
ImmunogenA synthesized peptide derived from human TTC11/FIS1.
SequenceEmail for sequence
Gene ID51024
Swiss protQ9Y3D6
SynonymsCGI-135; TTC11; FIS1; TTC11 / FIS1; fission
Calculated MW15kDa
Observed MW15KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, HepG2, 293T, NIH/3T3, Mouse liver, Rat liver
Cellular locationMitochondrion outer membrane, Peroxisome membrane, Single-pass membrane protein
Customer validation

WB(Mus musculus)

ABclonal:Western blot - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666) }

Western blot - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Western blot analysis of extracts of various cell lines, using TTC11/FIS1 antibody (A19666) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666) }

Western blot - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Western blot analysis of extracts from normal (control) and TTC11/FIS1 knockout (KO) HeLa cells, using TTC11/FIS1 antibody (A19666) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666) }

Immunohistochemistry - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Immunohistochemistry of paraffin-embedded rat brain using TTC11/FIS1 antibody (A19666) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666) }

Immunohistochemistry - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Immunohistochemistry of paraffin-embedded human lung cancer using TTC11/FIS1 antibody (A19666) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666) }

Immunohistochemistry - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Immunohistochemistry of paraffin-embedded mouse kidney using TTC11/FIS1 antibody (A19666) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666) }

Immunofluorescence - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Immunofluorescence analysis of NIH-3T3 cells using TTC11/FIS1 antibody (A19666). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666) }

Immunoprecipitation - [KO Validated] TTC11/FIS1 Rabbit mAb (A19666)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug TTC11/FIS1 antibody (A19666). Western blot was performed from the immunoprecipitate using TTC11/FIS1 antibody (A19666) at a dilition of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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