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TRAF2 Rabbit pAb (A0962)

Publications (11) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - TRAF2 Rabbit pAb (A0962)

Western blot analysis of extracts of various cell lines, using TRAF2 antibody (A0962) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - TRAF2 Rabbit pAb (A0962)

Immunohistochemistry of paraffin-embedded mouse brain using [KO Validated] TRAF2 Rabbit pAb (A0962) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TRAF2 Rabbit pAb (A0962)

Immunohistochemistry of paraffin-embedded mouse spleen using [KO Validated] TRAF2 Rabbit pAb (A0962) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TRAF2 Rabbit pAb (A0962)

Immunohistochemistry of paraffin-embedded rat spleen using [KO Validated] TRAF2 Rabbit pAb (A0962) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TRAF2 Rabbit pAb (A0962)

Immunohistochemistry of paraffin-embedded rat testis using [KO Validated] TRAF2 Rabbit pAb (A0962) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameTRAF2 Rabbit pAb
Catalog No.A0962
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TRAF2 (NP_066961.2).
SequenceMAAASVTPPGSLELLQPGFSKTLLGTKLEAKYLCSACRNVLRRPFQAQCGHRYCSFCLASILSSGPQNCAACVHEGIYEEGISILESSSAFPDNAARREVESLPAVCPSDGCTWKGTLKEYESCHEGRCPLMLTECPACKGLVRLGEKERHLEHECPERSLSCRHCRAPCCGADVKAHHEVCPKFPLTCDGCGKKKIPREKFQDHVKTCGKCRVPCRFHAIGCLETVEGEKQQEHEVQWLREHLAMLLSSVLEAKPLLGDQSHAGSELLQRCESLEKKTATFENIVCVLNREVERVAMTA
Gene ID7186
Swiss protQ12933
SynonymsMGC:45012; TRAP; TRAP3; TRAF2
Calculated MW53kDa/54kDa/55kDa/61kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesHeLa, 293T, THP-1, PC-12, Ramos, Jurkat, NIH/3T3
Cellular locationCytoplasm
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus)

FC(Homo sapiens)

ABclonal:Western blot - TRAF2 Rabbit pAb (A0962)}

Western blot - TRAF2 Rabbit pAb (A0962)

Western blot analysis of extracts of various cell lines, using TRAF2 antibody (A0962) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - TRAF2 Rabbit pAb (A0962)}

Immunohistochemistry - TRAF2 Rabbit pAb (A0962)

Immunohistochemistry of paraffin-embedded mouse brain using [KO Validated] TRAF2 Rabbit pAb (A0962) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TRAF2 Rabbit pAb (A0962)}

Immunohistochemistry - TRAF2 Rabbit pAb (A0962)

Immunohistochemistry of paraffin-embedded mouse spleen using [KO Validated] TRAF2 Rabbit pAb (A0962) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TRAF2 Rabbit pAb (A0962)}

Immunohistochemistry - TRAF2 Rabbit pAb (A0962)

Immunohistochemistry of paraffin-embedded rat spleen using [KO Validated] TRAF2 Rabbit pAb (A0962) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TRAF2 Rabbit pAb (A0962)}

Immunohistochemistry - TRAF2 Rabbit pAb (A0962)

Immunohistochemistry of paraffin-embedded rat testis using [KO Validated] TRAF2 Rabbit pAb (A0962) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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