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TRAF2 Rabbit pAb (A0962)

Tested applications:WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIPReactivity:Human, Mouse, Rat

Western blot analysis of extracts of various cell lines, using TRAF2 antibody (A0962) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - TRAF2 Rabbit pAb (A0962)

Immunohistochemistry of paraffin-embedded mouse brain using [KO Validated] TRAF2 Rabbit pAb (A0962) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse spleen using [KO Validated] TRAF2 Rabbit pAb (A0962) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded rat spleen using [KO Validated] TRAF2 Rabbit pAb (A0962) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded rat testis using [KO Validated] TRAF2 Rabbit pAb (A0962) at dilution of 1:250 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product name	TRAF2 Rabbit pAb
Catalog No.	A0962
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TRAF2 (NP_066961.2).
Sequence	MAAASVTPPGSLELLQPGFSKTLLGTKLEAKYLCSACRNVLRRPFQAQCGHRYCSFCLASILSSGPQNCAACVHEGIYEEGISILESSSAFPDNAARREVESLPAVCPSDGCTWKGTLKEYESCHEGRCPLMLTECPACKGLVRLGEKERHLEHECPERSLSCRHCRAPCCGADVKAHHEVCPKFPLTCDGCGKKKIPREKFQDHVKTCGKCRVPCRFHAIGCLETVEGEKQQEHEVQWLREHLAMLLSSVLEAKPLLGDQSHAGSELLQRCESLEKKTATFENIVCVLNREVERVAMTA
Gene ID	7186
Swiss prot	Q12933
Synonyms	MGC:45012; TRAP; TRAP3; TRAF2
Calculated MW	53kDa/54kDa/55kDa/61kDa
Observed MW	54kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key	Western blotting Immunohistochemistry Immunoprecipitation
Positive samples	HeLa, 293T, THP-1, PC-12, Ramos, Jurkat, NIH/3T3
Cellular location	Cytoplasm
Customer validation	WB(Homo sapiens, Mus musculus, Rattus norvegicus) FC(Homo sapiens)

Research Area

TRAF2 Rabbit pAb images

Western blot - TRAF2 Rabbit pAb (A0962)

Immunohistochemistry - TRAF2 Rabbit pAb (A0962)

Product Protocols

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References for TRAF2 Rabbit pAb (A0962)

Product:

TRAF2 Rabbit pAb

Journal:

Journal of Cellular And Molecular Medicine

Application:

IF:

5.31

Species:

Homo sapiens

PMID:

29266867

Title:

Combination of arsenic trioxide and Dasatinib: a new strategy to treat Philadelphia chromosome-positive acute lymphoblastic leukaemia

References for TRAF2 Rabbit pAb (A0962)

Product:

TRAF2 Rabbit pAb

Journal:

Toxicology and Applied Pharmacology

Application:

IF:

4.219

Species:

Mus musculus,Rattus norvegicus

PMID:

30240693

Title:

Berberine modulates ASK1 signaling mediated through TLR4/TRAF2 via upregulation of miR-23a

References for TRAF2 Rabbit pAb (A0962)

Product:

TRAF2 Rabbit pAb

Journal:

Journal of Cellular Physiology

Application:

IF:

6.384

Species:

Rattus norvegicus

PMID:

32372426

Title:

miR-506-3p alleviates uncontrolled osteoclastogenesis via repression of RANKL/NFATc1 signaling pathway

References for TRAF2 Rabbit pAb (A0962)

Product:

TRAF2 Rabbit pAb

Journal:

Microb Pathog

Application:

IF:

3.738

Species:

Homo sapiens

PMID:

32525021

Title:

Giardia duodenalis induces extrinsic pathway of apoptosis in intestinal epithelial cells through activation of TNFR1 and K63 de-ubiquitination of RIP1 in vitro

References for TRAF2 Rabbit pAb (A0962)

Product:

TRAF2 Rabbit pAb

Journal:

Chem Biol Interact

Application:

IF:

3.922

Species:

Mus musculus

PMID:

32971123

Title:

Ellagic acid blocks RANKL–RANK interaction and suppresses RANKL-induced osteoclastogenesis by inhibiting RANK signaling pathways

References for TRAF2 Rabbit pAb (A0962)

Product:

TRAF2 Rabbit pAb

Journal:

Inflammation

Application:

IF:

4.092

Species:

Homo sapiens

PMID:

32876895

Title:

MicroRNA-532-3p Regulates Pro-Inflammatory Human THP-1 Macrophages by Targeting ASK1/p38 MAPK Pathway

References for TRAF2 Rabbit pAb (A0962)

Product:

TRAF2 Rabbit pAb

Journal:

Food Funct

Application:

IF:

5.396

Species:

Mus musculus

PMID:

33057520

Title:

Acanthopanax senticosus aqueous extract ameliorates ovariectomy-induced bone loss in middle-aged mice by inhibiting the receptor activator of nuclear factor-κB ligand-induced osteoclastogenesis

References for TRAF2 Rabbit pAb (A0962)

Product:

TRAF2 Rabbit pAb

Journal:

Int Immunopharmacol

Application:

IF:

4.932

Species:

Mus musculus

PMID:

33677256

Title:

(-)-Epigallocatechin-3-gallate inhibits osteoclastogenesis by blocking RANKL-RANK interaction and suppressing NF-κB and MAPK signaling pathways

References for TRAF2 Rabbit pAb (A0962)

Product:

TRAF2 Rabbit pAb

Journal:

Oxidative medicine and cellular longevity

Application:

IF:

6.54

Species:

Mus musculus

PMID:

35096272

Title:

The Role of CD147 in Pathological Cardiac Hypertrophy Is Regulated by Glycosylation

References for TRAF2 Rabbit pAb (A0962)

Product:

TRAF2 Rabbit pAb

Journal:

Adipocyte

Application:

IF:

4.53

Species:

Mus musculus

PMID:

34311651

Title:

microRNA-26a shuttled by extracellular vesicles secreted from adipose-derived mesenchymal stem cells reduce neuronal damage through KLF9-mediated regulation of TRAF2/KLF2 axis

References for TRAF2 Rabbit pAb (A0962)

Product:

TRAF2 Rabbit pAb

Journal:

Journal of biochemical and molecular toxicology

Application:

IF:

Species:

Mus musculus

PMID:

33754447

Title: