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[KO Validated] TRAF2 Polyclonal Antibody (A0962)

Publications (2) Datasheet

ABclonal:Western blot - [KO Validated] TRAF2 Polyclonal Antibody (A0962)
ABclonal:Western blot - [KO Validated] TRAF2 Polyclonal Antibody (A0962)
ABclonal:Immunofluorescence - [KO Validated] TRAF2 Polyclonal Antibody (A0962)

Overview

Product name[KO Validated] TRAF2 Polyclonal Antibody
Catalog No.A0962
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TRAF2 (NP_066961.2).
SequenceMAAASVTPPGSLELLQPGFSKTLLGTKLEAKYLCSACRNVLRRPFQAQCGHRYCSFCLASILSSGPQNCAACVHEGIYEEGISILESSSAFPDNAARREVESLPAVCPSDGCTWKGTLKEYESCHEGRCPLMLTECPACKGLVRLGEKERHLEHECPERSLSCRHCRAPCCGADVKAHHEVCPKFPLTCDGCGKKKIPREKFQDHVKTCGKCRVPCRFHAIGCLETVEGEKQQEHEVQWLREHLAMLLSSVLEAKPLLGDQSHAGSELLQRCESLEKKTATFENIVCVLNREVERVAMTA
Gene ID7186
Swiss protQ12933
SynonymsTRAF2; MGC:45012; TRAP; TRAP3; TNF receptor-associated factor 2
Calculated MW53kDa/54kDa/55kDa/61kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, 293T, THP-1, PC-12, Ramos, Jurkat, NIH/3T3
Cellular locationCytoplasm
Customer validation

WB(Human, Mouse, Rat)

ABclonal:Western blot - [KO Validated] TRAF2 Polyclonal Antibody (A0962) }

Western blot - [KO Validated] TRAF2 Polyclonal Antibody (A0962)

Western blot analysis of extracts of various cell lines, using TRAF2 antibody (A0962) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] TRAF2 Polyclonal Antibody (A0962) }

Western blot - [KO Validated] TRAF2 Polyclonal Antibody (A0962)

Western blot analysis of extracts from normal (control) and TRAF2 knockout (KO) 293T cells, using TRAF2 antibody (A0962) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 6s.
ABclonal:Immunofluorescence - [KO Validated] TRAF2 Polyclonal Antibody (A0962) }

Immunofluorescence - [KO Validated] TRAF2 Polyclonal Antibody (A0962)

Immunofluorescence analysis of HeLa cells using TRAF2 antibody (A0962). Blue: DAPI for nuclear staining.

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