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[KO Validated] TMPO Rabbit pAb (A2534)

Reviews (2) Datasheet

ABclonal:Western blot - [KO Validated] TMPO Polyclonal Antibody (A2534)
ABclonal:Western blot - [KO Validated] TMPO Polyclonal Antibody (A2534)
ABclonal:Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (A2534)
ABclonal:Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (A2534)
ABclonal:Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (A2534)
ABclonal:Immunofluorescence - [KO Validated] TMPO Polyclonal Antibody (A2534)
Review
ABclonal: review for [KO Validated] TMPO Rabbit pAb(A2534)
Review
ABclonal: review for [KO Validated] TMPO Rabbit pAb(A2534)

Overview

Product name[KO Validated] TMPO Rabbit pAb
Catalog No.A2534
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. Mutations in this gene are associated with dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-410 of human TMPO (NP_001027454.1).
SequenceMPEFLEDPSVLTKDKLKSELVANNVTLPAGEQRKDVYVQLYLQHLTARNRPPLPAGTNSKGPPDFSSDEEREPTPVLGSGAAAAGRSRAAVGRKATKKTDKPRQEDKDDLDVTELTNEDLLDQLVKYGVNPGPIVGTTRKLYEKKLLKLREQGTESRSSTPLPTISSSAENTRQNGSNDSDRYSDNEEDSKIELKLEKREPLKGRAKTPVTLKQRRVEHNQSYSQAGITETEWTSGSSKGGPLQALTRESTRGSRRTPRKRVETSEHFRIDGPVISESTPIAETIMASSNESLVVNRVTGNFKHASPILPITEFSDIPRRAPKKPLTRAEVGEKTEERRVERDILKEMFPYEASTPTGISASCRRPIKGAAGRPLELSDFRMEESFSSKYVPKYVPLADVKSEKTKKGRS
Gene ID7112
Swiss protP42166/P42167
SynonymsTMPO; CMD1T; LAP2; LEMD4; PRO0868; TP
Calculated MW38kDa/50kDa/75kDa
Observed MW51kDa/80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF7, 293T, HepG2, mouse liver, rat liver
Cellular locationChromosome, Nucleus
Customer validation

(Human lung cancer cell)

ABclonal:Western blot - [KO Validated] TMPO Polyclonal Antibody (A2534) }

Western blot - [KO Validated] TMPO Polyclonal Antibody (A2534)

Western blot analysis of extracts of various cell lines, using TMPO antibody (A2534) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] TMPO Polyclonal Antibody (A2534) }

Western blot - [KO Validated] TMPO Polyclonal Antibody (A2534)

Western blot analysis of extracts from normal (control) and TMPO knockout (KO) 293T cells, using TMPO antibody (A2534) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (A2534) }

Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (A2534)

Immunohistochemistry of paraffin-embedded rat brain using TMPO Antibody (A2534) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (A2534) }

Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (A2534)

Immunohistochemistry of paraffin-embedded human tonsil using TMPO Antibody (A2534) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (A2534) }

Immunohistochemistry - [KO Validated] TMPO Polyclonal Antibody (A2534)

Immunohistochemistry of paraffin-embedded mouse brain using TMPO Antibody (A2534) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] TMPO Polyclonal Antibody (A2534) }

Immunofluorescence - [KO Validated] TMPO Polyclonal Antibody (A2534)

Immunofluorescence analysis of U2OS cells using TMPO antibody (A2534).

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