Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

TBP Rabbit pAb (A2192)

Reviews (3) Publications (3) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - TBP Rabbit pAb (A2192)

Western blot analysis of various lysates, using TBP antibody (A2192) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Review

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Overview

Product name	TBP Rabbit pAb
Catalog No.	A2192
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 25-42, and expansion of the number of repeats to 45-66 increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TBP (NP_001165556.1).
Sequence	MTPGIPIFSPMMPYGTGLTPQPIQNTNSLSILEEQQRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQAVAAAAVQQSTSQQATQGTSGQAPQ
Gene ID	6908
Swiss prot	P20226
Synonyms	HDL4; TBP1; GTF2D; SCA17; TFIID; GTF2D1; TBP
Calculated MW	38kDa
Observed MW	33-38kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key	Western blotting
Positive samples	RAW264.7, C6
Cellular location	Nucleus
Customer validation	WB (Mouse macrophage, Homo sapiens, Mus musculus) IF (Mouse macrophage) IHC (Mouse macrophage)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Research Area & PathwaysEpigenetic & Nuclear SignalingTranscription Factors

TBP Rabbit pAb images

Western blot - TBP Rabbit pAb (A2192)

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Related Targets

Based on existing publications, the following genes are closely related to research on TBP. (Numbers indicate number of published articles where both targets appear.)

◀1/▶

Related Research Area

Based on existing publications, the following research topics are related to TBP. (Distance between topics and target gene indicate popularity.) TBP

* Data provided by citexs.com, for reference only.

References for TBP Rabbit pAb (A2192)

Product:

TBP Rabbit pAb

Journal:

Journal of Cancer

Application:

IF:

4.207

Species:

Homo sapiens

PMID: