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[KO Validated] TBP Rabbit pAb (A2192)

Reviews (3)Publications (2) Datasheet

ABclonal:Western blot - [KO Validated] TBP Polyclonal Antibody (A2192)
ABclonal:Western blot - [KO Validated] TBP Polyclonal Antibody (A2192)
Review
ABclonal: review for [KO Validated] TBP Rabbit pAb(A2192)
Review
ABclonal: review for [KO Validated] TBP Rabbit pAb(A2192)
Review
ABclonal: review for [KO Validated] TBP Rabbit pAb(A2192)

Overview

Product name[KO Validated] TBP Rabbit pAb
Catalog No.A2192
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 25-42, and expansion of the number of repeats to 45-66 increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human TBP (NP_001165556.1).
SequenceMTPGIPIFSPMMPYGTGLTPQPIQNTNSLSILEEQQRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQAVAAAAVQQSTSQQATQGTSGQAPQ
Gene ID6908
Swiss protP20226
SynonymsTBP; GTF2D; GTF2D1; HDL4; SCA17; TFIID
Calculated MW35kDa/37kDa
Observed MW43kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, MCF-7, K-562, 293T
Cellular locationNucleus
Customer validation

(Mouse macrophage)

WB(Homo sapiens)

ABclonal:Western blot - [KO Validated] TBP Polyclonal Antibody (A2192) }

Western blot - [KO Validated] TBP Polyclonal Antibody (A2192)

Western blot analysis of extracts of various cell lines, using TBP antibody (A2192) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] TBP Polyclonal Antibody (A2192) }

Western blot - [KO Validated] TBP Polyclonal Antibody (A2192)

Western blot analysis of extracts from normal (control) and TBP knockout (KO) HeLa cells, using TBP antibody (A2192) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

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