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[KO Validated] TAGLN2 Rabbit pAb (A6761)

Datasheet

ABclonal:Western blot - [KO Validated] TAGLN2 Rabbit pAb (A6761)
ABclonal:Western blot - [KO Validated] TAGLN2 Rabbit pAb (A6761)
ABclonal:Immunofluorescence - [KO Validated] TAGLN2 Rabbit pAb (A6761)

Overview

Product name[KO Validated] TAGLN2 Rabbit pAb
Catalog No.A6761
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is similar to the protein transgelin, which is one of the earliest markers of differentiated smooth muscle. The specific function of this protein has not yet been determined, although it is thought to be a tumor suppressor. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human TAGLN2 (NP_003555.1).
SequenceMANRGPAYGLSREVQQKIEKQYDADLEQILIQWITTQCRKDVGRPQPGRENFQNWLKDGTVLCELINALYPEGQAPVKKIQASTMAFKQMEQISQFLQAAERYGINTTDIFQTVDLWEGKNMACVQRTLMNLGGLAVARDDGLFSGDPNWFPKKSKENPRNFSDNQLQEGKNVIGLQMGTNRGASQAGMTGYGMPRQIL
Gene ID8407
Swiss protP37802
SynonymsTAGLN2; HA1756
Calculated MW22kDa/24kDa
Observed MW22KDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa, A-549, Mouse heart
Cellular location
ABclonal:Western blot - [KO Validated] TAGLN2 Rabbit pAb (A6761) }

Western blot - [KO Validated] TAGLN2 Rabbit pAb (A6761)

Western blot analysis of extracts from normal (control) and TAGLN2 knockout (KO) 293T cells, using TAGLN2 antibody (A6761) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] TAGLN2 Rabbit pAb (A6761) }

Western blot - [KO Validated] TAGLN2 Rabbit pAb (A6761)

Western blot analysis of extracts of various cell lines, using TAGLN2 antibody (A6761) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunofluorescence - [KO Validated] TAGLN2 Rabbit pAb (A6761) }

Immunofluorescence - [KO Validated] TAGLN2 Rabbit pAb (A6761)

Immunofluorescence analysis of BALB-3T3 cells using [KO Validated] TAGLN2 Rabbit pAb (A6761) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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