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[KO Validated] Smad3 Rabbit mAb (A19115)

Publications (4) Datasheet

ABclonal:Western blot - [KO Validated] Smad3 Rabbit mAb (A19115)

Western blot analysis of extracts from normal (control) and Smad3 knockout (KO) 293T cells, using Smad3 antibody (A19115) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] Smad3 Rabbit mAb (A19115)

Western blot analysis of extracts of various cell lines, using Smad3 antibody (A19115) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] Smad3 Rabbit mAb (A19115)

Immunohistochemistry of paraffin-embedded human liver using Smad3 Rabbit mAb (A19115) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] Smad3 Rabbit mAb (A19115)

Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Smad3 Rabbit mAb (A19115) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] Smad3 Rabbit mAb (A19115)

Immunoprecipitation analysis of 300ug extracts of A-431 cells using 3ug Smad3 antibody (A19115). Western blot was performed from the immunoprecipitate using Smad3 antibody (A19115) at a dilition of 1:500.

Overview

Product name[KO Validated] Smad3 Rabbit mAb
Catalog No.A19115
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
ImmunogenA synthesized peptide derived from human Smad3
SequenceEmail for sequence
Gene ID4088
Swiss protP84022
SynonymsHSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3; SMAD3
Calculated MW48kDa
Observed MW58kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesA-431, Mouse brain, Rat brain, 293T
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mus musculus)

ABclonal:Western blot - [KO Validated] Smad3 Rabbit mAb (A19115) }

Western blot - [KO Validated] Smad3 Rabbit mAb (A19115)

Western blot analysis of extracts from normal (control) and Smad3 knockout (KO) 293T cells, using Smad3 antibody (A19115) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] Smad3 Rabbit mAb (A19115) }

Western blot - [KO Validated] Smad3 Rabbit mAb (A19115)

Western blot analysis of extracts of various cell lines, using Smad3 antibody (A19115) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] Smad3 Rabbit mAb (A19115) }

Immunohistochemistry - [KO Validated] Smad3 Rabbit mAb (A19115)

Immunohistochemistry of paraffin-embedded human liver using Smad3 Rabbit mAb (A19115) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - [KO Validated] Smad3 Rabbit mAb (A19115) }

Immunofluorescence - [KO Validated] Smad3 Rabbit mAb (A19115)

Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Smad3 Rabbit mAb (A19115) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] Smad3 Rabbit mAb (A19115) }

Immunoprecipitation - [KO Validated] Smad3 Rabbit mAb (A19115)

Immunoprecipitation analysis of 300ug extracts of A-431 cells using 3ug Smad3 antibody (A19115). Western blot was performed from the immunoprecipitate using Smad3 antibody (A19115) at a dilition of 1:500.

* For research use only. Not for therapeutic or diagnostic purposes.

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