Products > Catalog Antibodies > Monoclonal Antibodies

[KO Validated] Smad3 Rabbit mAb (A19115)

Publications (5) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] Smad3 Rabbit mAb (A19115)

Western blot analysis of extracts from wild type (WT) and Smad3 knockout (KO) 293T cells, using Smad3 antibody (A19115) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] Smad3 Rabbit mAb (A19115)

Western blot analysis of extracts of A-431 cells, using Smad3 antibody (A19115) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - [KO Validated] Smad3 Rabbit mAb (A19115)

Immunohistochemistry of paraffin-embedded human liver using Smad3 Rabbit mAb (A19115) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] Smad3 Rabbit mAb (A19115)

Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Smad3 Rabbit mAb (A19115) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] Smad3 Rabbit mAb (A19115)

Immunoprecipitation analysis of 300ug extracts of A-431 cells using 3ug Smad3 antibody (A19115). Western blot was performed from the immunoprecipitate using Smad3 antibody (A19115) at a dilition of 1:500.

Overview

Product name[KO Validated] Smad3 Rabbit mAb
Catalog No.A19115
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of human Smad3 (P84022).
SequenceMDAGSPNLSPNPMSPAHNNLDLQPVTYCEPAFWCSISYYELNQRVGETFHASQPSMTVDGFTDPSNSERFCLGLLSNVNRNAAVELTRRHIGRGVRLYYIG
Gene ID4088
Swiss protP84022
SynonymsHSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3; SMAD3
Calculated MW48kDa
Observed MW52kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesA-431, 293T
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mus musculus, Rattus norvegicus)

ABclonal:Western blot - [KO Validated] Smad3 Rabbit mAb (A19115)}

Western blot - [KO Validated] Smad3 Rabbit mAb (A19115)

Western blot analysis of extracts from wild type (WT) and Smad3 knockout (KO) 293T cells, using Smad3 antibody (A19115) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] Smad3 Rabbit mAb (A19115)}

Western blot - [KO Validated] Smad3 Rabbit mAb (A19115)

Western blot analysis of extracts of A-431 cells, using Smad3 antibody (A19115) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - [KO Validated] Smad3 Rabbit mAb (A19115)}

Immunohistochemistry - [KO Validated] Smad3 Rabbit mAb (A19115)

Immunohistochemistry of paraffin-embedded human liver using Smad3 Rabbit mAb (A19115) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - [KO Validated] Smad3 Rabbit mAb (A19115)}

Immunofluorescence - [KO Validated] Smad3 Rabbit mAb (A19115)

Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Smad3 Rabbit mAb (A19115) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] Smad3 Rabbit mAb (A19115)}

Immunoprecipitation - [KO Validated] Smad3 Rabbit mAb (A19115)

Immunoprecipitation analysis of 300ug extracts of A-431 cells using 3ug Smad3 antibody (A19115). Western blot was performed from the immunoprecipitate using Smad3 antibody (A19115) at a dilition of 1:500.

Inquire About This Product

Submit your question about A19115 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A19115? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order