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[KO Validated] SUZ12 Rabbit pAb (A7786)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] SUZ12 Rabbit pAb (A7786)
ABclonal:Western blot - [KO Validated] SUZ12 Rabbit pAb (A7786)
ABclonal:Immunohistochemistry - [KO Validated] SUZ12 Rabbit pAb (A7786)
ABclonal:Immunohistochemistry - [KO Validated] SUZ12 Rabbit pAb (A7786)
ABclonal:Immunohistochemistry - [KO Validated] SUZ12 Rabbit pAb (A7786)
ABclonal:Immunofluorescence - [KO Validated] SUZ12 Rabbit pAb (A7786)
ABclonal:Immunoprecipitation - [KO Validated] SUZ12 Rabbit pAb (A7786)

Overview

Product name[KO Validated] SUZ12 Rabbit pAb
Catalog No.A7786
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 470-739 of human SUZ12 (NP_056170.2).
SequenceCHSRFIFNYVYHPKGARIDVSINECYDGSYAGNPQDIHRQPGFAFSRNGPVKRTPITHILVCRPKRTKASMSEFLESEDGEVEQQRTYSSGHNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIEEFSDVNEGEKEVMKLWNLHVMKHGFIADNQMNHACMLFVENYGQKIIKKNLCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQQKLEKGESASPANEEITEEQNGTANGFSEINSKEKALETDSVSGVSKQSKKQKL
Gene ID23512
Swiss protQ15022
SynonymsSUZ12; CHET9; JJAZ1
Calculated MW83kDa
Observed MW83kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, BT-474, MCF-7, 293T
Cellular locationNucleus
Customer validation

WB(Homo sapiens)

IP(Homo sapiens)

ABclonal:Western blot - [KO Validated] SUZ12 Rabbit pAb (A7786) }

Western blot - [KO Validated] SUZ12 Rabbit pAb (A7786)

Western blot analysis of extracts of various cell lines, using SUZ12 Antibody (A7786) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] SUZ12 Rabbit pAb (A7786) }

Western blot - [KO Validated] SUZ12 Rabbit pAb (A7786)

Western blot analysis of extracts from normal (control) and SUZ12 knockout (KO) HeLa cells, using SUZ12 antibody (A7786) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] SUZ12 Rabbit pAb (A7786) }

Immunohistochemistry - [KO Validated] SUZ12 Rabbit pAb (A7786)

Immunohistochemistry of paraffin-embedded rat brain using SUZ12 antibody (A7786) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] SUZ12 Rabbit pAb (A7786) }

Immunohistochemistry - [KO Validated] SUZ12 Rabbit pAb (A7786)

Immunohistochemistry of paraffin-embedded human gastric cancer using SUZ12 antibody (A7786) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] SUZ12 Rabbit pAb (A7786) }

Immunohistochemistry - [KO Validated] SUZ12 Rabbit pAb (A7786)

Immunohistochemistry of paraffin-embedded mouse brain using SUZ12 antibody (A7786) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] SUZ12 Rabbit pAb (A7786) }

Immunofluorescence - [KO Validated] SUZ12 Rabbit pAb (A7786)

Immunofluorescence analysis of C6 cells using SUZ12 Polyclonal Antibody (A7786) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] SUZ12 Rabbit pAb (A7786) }

Immunoprecipitation - [KO Validated] SUZ12 Rabbit pAb (A7786)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SUZ12 antibody (A7786). Western blot was performed from the immunoprecipitate using SUZ12 antibody (A7786) at a dilition of 1:500.

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