Products > Catalog Antibodies > Monoclonal Antibodies

[KO Validated] STAT3 Rabbit mAb (A19566)

Publications (6) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] STAT3 Rabbit mAb (A19566)

Western blot analysis of extracts from wild type (WT) and STAT3 knockout (KO) HeLa cells, using STAT3 antibody (A19566) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

ABclonal:Western blot - [KO Validated] STAT3 Rabbit mAb (A19566)

Western blot analysis of extracts of various cell lines, using STAT3 antibody (A19566) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.

ABclonal:Western blot - [KO Validated] STAT3 Rabbit mAb (A19566)

Western blot analysis of extracts of various cell lines, using STAT3 antibody (A19566) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - [KO Validated] STAT3 Rabbit mAb (A19566)

Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] STAT3 Rabbit mAb (A19566) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - [KO Validated] STAT3 Rabbit mAb (A19566)

Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] STAT3 Rabbit mAb (A19566) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] STAT3 Rabbit mAb (A19566)

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] STAT3 Rabbit mAb (A19566) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] STAT3 Rabbit mAb (A19566)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug STAT3 antibody (A19566). Western blot was performed from the immunoprecipitate using STAT3 antibody (A19566) at a dilition of 1:1000.

Overview

Product name[KO Validated] STAT3 Rabbit mAb
Catalog No.A19566
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 600-700 of human STAT3 (P40763).
SequenceTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPG
Gene ID6774
Swiss protP40763
SynonymsADMIO; ADMIO1; APRF; HIES; STAT3; Stat3
Calculated MW88kDa
Observed MW82KDa/88KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesA-549, 293T, Mouse thymus, Mouse kidney, Rat brain, HeLa
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Homo sapiens, Mus musculus)

IF(Homo sapiens)

ABclonal:Western blot - [KO Validated] STAT3 Rabbit mAb (A19566)}

Western blot - [KO Validated] STAT3 Rabbit mAb (A19566)

Western blot analysis of extracts from wild type (WT) and STAT3 knockout (KO) HeLa cells, using STAT3 antibody (A19566) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.
ABclonal:Western blot - [KO Validated] STAT3 Rabbit mAb (A19566)}

Western blot - [KO Validated] STAT3 Rabbit mAb (A19566)

Western blot analysis of extracts of various cell lines, using STAT3 antibody (A19566) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.
ABclonal:Western blot - [KO Validated] STAT3 Rabbit mAb (A19566)}

Western blot - [KO Validated] STAT3 Rabbit mAb (A19566)

Western blot analysis of extracts of various cell lines, using STAT3 antibody (A19566) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunohistochemistry - [KO Validated] STAT3 Rabbit mAb (A19566)}

Immunohistochemistry - [KO Validated] STAT3 Rabbit mAb (A19566)

Immunohistochemistry of paraffin-embedded human breast cancer using [KO Validated] STAT3 Rabbit mAb (A19566) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - [KO Validated] STAT3 Rabbit mAb (A19566)}

Immunohistochemistry - [KO Validated] STAT3 Rabbit mAb (A19566)

Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] STAT3 Rabbit mAb (A19566) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - [KO Validated] STAT3 Rabbit mAb (A19566)}

Immunofluorescence - [KO Validated] STAT3 Rabbit mAb (A19566)

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] STAT3 Rabbit mAb (A19566) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] STAT3 Rabbit mAb (A19566)}

Immunoprecipitation - [KO Validated] STAT3 Rabbit mAb (A19566)

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug STAT3 antibody (A19566). Western blot was performed from the immunoprecipitate using STAT3 antibody (A19566) at a dilition of 1:1000.

Inquire About This Product

Submit your question about A19566 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A19566? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order