Products > Catalog Antibodies > Polyclonal Antibodies

[KO Validated] STAT3 Polyclonal Antibody (A1192)

Reviews (3)Publications (3) Datasheet

ABclonal:Western blot - [KO Validated] STAT3 Polyclonal Antibody (A1192)
ABclonal:Western blot - [KO Validated] STAT3 Polyclonal Antibody (A1192)
ABclonal:Immunofluorescence - [KO Validated] STAT3 Polyclonal Antibody (A1192)
ABclonal:Immunofluorescence - [KO Validated] STAT3 Polyclonal Antibody (A1192)
Review
ABclonal:Western blot (WB) review for [KO Validated] STAT3 Polyclonal Antibody(A1192)
Review
ABclonal:Immunohistochemistry (IHC) review for [KO Validated] STAT3 Polyclonal Antibody(A1192)
Review
ABclonal:Western blot (WB) review for [KO Validated] STAT3 Polyclonal Antibody(A1192)

Overview

Product name[KO Validated] STAT3 Polyclonal Antibody
Catalog No.A1192
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 640-770 of human STAT3 (NP_644805.1).
SequenceYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATSPM
Gene ID6774
Swiss protP40763
SynonymsSTAT3; ADMIO; ADMIO1; APRF; HIES; signal transducer and activator of transcription 3
Calculated MW83kDa/87kDa/88kDa
Observed MW90kDa
ReactivityHuman, Mouse, Rat, Monkey
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293T
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Human breast cancer, PK15, Mouse)

IHC(Human breast cancer)

ABclonal:Western blot - [KO Validated] STAT3 Polyclonal Antibody (A1192) }

Western blot - [KO Validated] STAT3 Polyclonal Antibody (A1192)

Western blot analysis of extracts of various cell lines, using STAT3 antibody (A1192) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] STAT3 Polyclonal Antibody (A1192) }

Western blot - [KO Validated] STAT3 Polyclonal Antibody (A1192)

Western blot analysis of extracts from normal (control) and STAT3 knockout (KO) HeLa cells, using STAT3 antibody (A1192) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - [KO Validated] STAT3 Polyclonal Antibody (A1192) }

Immunofluorescence - [KO Validated] STAT3 Polyclonal Antibody (A1192)

Immunofluorescence analysis of rat brain using STAT3 antibody (A1192) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] STAT3 Polyclonal Antibody (A1192) }

Immunofluorescence - [KO Validated] STAT3 Polyclonal Antibody (A1192)

Immunofluorescence analysis of mouse brain using STAT3 antibody (A1192) at dilution of 1:100. Blue: DAPI for nuclear staining.

Submit an review for A1192

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A1192 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A1192? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
Add to Cart

or Request quote for bulk purchase

Available for next day delivery (US only)

Contact us to order