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SQSTM1/p62 Rabbit pAb (A11483)

Tested applications:WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIPReactivity:Human, Mouse, Rat

Western blot analysis of extracts of various cell lines, using SQSTM1/p62 antibody (A11483) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A11483)

Immunohistochemistry of paraffin-embedded Rat ovary using SQSTM1/p62 antibody (A11483) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded Human appendix using SQSTM1/p62 antibody (A11483) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded Mouse liver using SQSTM1/p62 antibody (A11483) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - SQSTM1/p62 Rabbit pAb (A11483)

Immunofluorescence analysis of U2OS cells using SQSTM1/p62 antibody (A11483) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - SQSTM1/p62 Rabbit pAb (A11483)

Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug SQSTM1/p62 antibody (A11483). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (A11483) at a dilition of 1:1000.

Overview

Product name	SQSTM1/p62 Rabbit pAb
Catalog No.	A11483
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1/p62 (NP_001135770.1).
Sequence	MAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL
Gene ID	8878
Swiss prot	Q13501
Synonyms	SQSTM1; A170; DMRV; FTDALS3; NADGP; OSIL; PDB3; ZIP3; p60; p62; p62B
Calculated MW	38kDa/47kDa
Observed MW	62KDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC ICC IF IP ChIP ChIP-seq RIP FC ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:20 - 1:50
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	293T, HeLa
Cellular location	Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Late endosome, Lysosome, Nucleus, P-body, autophagosome
Customer validation	WB(Rattus norvegicus, Homo sapiens, Mus musculus)

Research Area

ResearchCell Biology & Developmental BiologyAutophagyMitochondrial Control of Autophagy

SQSTM1/p62 Rabbit pAb images

Western blot - SQSTM1/p62 Rabbit pAb (A11483)

Immunohistochemistry - SQSTM1/p62 Rabbit pAb (A11483)

Immunofluorescence - SQSTM1/p62 Rabbit pAb (A11483)

Immunofluorescence analysis of U2OS cells using SQSTM1/p62 antibody (A11483) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation - SQSTM1/p62 Rabbit pAb (A11483)

Product Protocols

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References for SQSTM1/p62 Rabbit pAb (A11483)

Product:

SQSTM1/p62 Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

4.932

Species:

Rattus norvegicus

PMID:

30959368

Title:

Genipin protects rats against lipopolysaccharide-induced acute lung injury by reinforcing autophagy.

References for SQSTM1/p62 Rabbit pAb (A11483)

Product:

SQSTM1/p62 Rabbit pAb

Journal:

Journal of Natural Products

Application:

IF:

4.05

Species:

Homo sapiens

PMID:

32356659

Title:

Cytotoxic Monoterpenoid Indole Alkaloids from Tabernaemontana corymbosa as Potent Autophagy Inhibitors by the Attenuation of Lysosomal Acidification

References for SQSTM1/p62 Rabbit pAb (A11483)

Product:

SQSTM1/p62 Rabbit pAb

Journal:

Frontiers in Microbiology

Application:

IF:

5.64

Species:

Homo sapiens

PMID:

31849860

Title:

Transmembrane Protein 39A Promotes the Replication of Encephalomyocarditis Virus via Autophagy Pathway.

References for SQSTM1/p62 Rabbit pAb (A11483)

Product:

SQSTM1/p62 Rabbit pAb

Journal:

Acta Pharmacol Sin

Application:

IF:

6.15

Species:

Mus musculus

PMID:

32796955

Title:

Baicalein attenuates cardiac hypertrophy in mice via suppressing oxidative stress and activating autophagy in cardiomyocytes

References for SQSTM1/p62 Rabbit pAb (A11483)

Product:

SQSTM1/p62 Rabbit pAb

Journal:

Exp Ther Med

Application:

IF:

2.44

Species:

Homo sapiens

PMID:

33850531

Title:

Long non-coding RNA ADAMTS9-AS2 inhibits liver cancer cell proliferation, migration and invasion

References for SQSTM1/p62 Rabbit pAb (A11483)

Product:

SQSTM1/p62 Rabbit pAb

Journal:

Inflammopharmacology

Application:

IF:

4.47

Species:

Homo sapiens

PMID:

34085175

Title:

Exogenous stromal cell-derived factor-1 (SDF-1) suppresses the NLRP3 inflammasome and inhibits pyroptosis in synoviocytes from osteoarthritic joints via activation of the AMPK signaling pathway

References for SQSTM1/p62 Rabbit pAb (A11483)

Product:

SQSTM1/p62 Rabbit pAb

Journal:

Autophagy

Application:

IF:

16.01

Species:

Homo sapiens

PMID:

33719859

Title:

Selective autophagy receptor SQSTM1/ p62 inhibits Seneca Valley virus replication by targeting viral VP1 and VP3

References for SQSTM1/p62 Rabbit pAb (A11483)

Product:

SQSTM1/p62 Rabbit pAb

Journal:

Int Immunopharmacol

Application:

IF:

4.932

Species:

Rattus norvegicus

PMID:

34537481

Title:

6-Gingerol protects against cerebral ischemia/reperfusion injury by inhibiting NLRP3 inflammasome and apoptosis via TRPV1/FAF1 complex dissociation-mediated autophagy

References for SQSTM1/p62 Rabbit pAb (A11483)

Product:

SQSTM1/p62 Rabbit pAb

Journal:

Nat Commun

Application:

IF:

14.919

Species:

Rattus norvegicus

PMID:

34815412