Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

[KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

KO/KDValidated

Publications (9) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Rat

ABclonal:Western blot - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Western blot analysis of various lysates using [KO Validated] SQSTM1/p62 Rabbit pAb (A11250) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Western blot analysis of lysates from Jurkat cells, using [KO Validated] SQSTM1/p62 Rabbit pAb (A11250) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Western blot analysis of lysates from wild type(WT) and SQSTM1/p62 knockout (KO) 293T(KO) cells, using [KO Validated] SQSTM1/p62 Rabbit pAb (A11250) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Immunohistochemistry analysis of SQSTM1/p62 in paraffin-embedded human colon carcinoma using [KO Validated] SQSTM1/p62 Rabbit pAb (A11250) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Immunofluorescence analysis of U2OS cells using [KO Validated] SQSTM1/p62 Rabbit pAb (A11250) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Immunoprecipitation analysis of 200 μg extracts of C6 cells using 3 μg SQSTM1/p62 antibody (A11250). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (A11250) at a dilution of 1:1000.

You may also interested in:

Overview

Product name [KO Validated] SQSTM1/p62 Rabbit pAb
Catalog No. A11250
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1/p62 (NP_003891.1).
Sequence MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL
Gene ID 8878
Swiss prot Q13501
Synonyms p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3; 62
Calculated MW 48kDa
Observed MW 62kDa

Applications

Reactivity Human, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • IHC-P 1:20 - 1:50
  • IF/ICC 1:20 - 1:50
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunoprecipitation    Immunohistochemistry    Immunofluorescence    
Positive samples 293T, Jurkat
Cellular location Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Late endosome, Lysosome, Nucleus, P-body, autophagosome
Customer validation

WB (Bombyx mori, Homo sapiens, Mus musculus, Rattus norvegicus)

IHC (Rattus norvegicus, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] SQSTM1/p62 Rabbit pAb images

ABclonal:Western blot - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)}

Western blot - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Western blot analysis of various lysates using [KO Validated] SQSTM1/p62 Rabbit pAb (A11250) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)}

Western blot - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Western blot analysis of lysates from Jurkat cells, using [KO Validated] SQSTM1/p62 Rabbit pAb (A11250) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)}

Western blot - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Western blot analysis of lysates from wild type(WT) and SQSTM1/p62 knockout (KO) 293T(KO) cells, using [KO Validated] SQSTM1/p62 Rabbit pAb (A11250) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)}

Immunohistochemistry - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Immunohistochemistry analysis of SQSTM1/p62 in paraffin-embedded human colon carcinoma using [KO Validated] SQSTM1/p62 Rabbit pAb (A11250) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)}

Immunofluorescence - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Immunofluorescence analysis of U2OS cells using [KO Validated] SQSTM1/p62 Rabbit pAb (A11250) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)}

Immunoprecipitation - [KO Validated] SQSTM1/p62 Rabbit pAb (A11250)

Immunoprecipitation analysis of 200 μg extracts of C6 cells using 3 μg SQSTM1/p62 antibody (A11250). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (A11250) at a dilution of 1:1000.

Inquire About This Product

Submit your question about A11250 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on SQSTM1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SQSTM1. (Distance between topics and target gene indicate popularity.) SQSTM1

* Data provided by citexs.com, for reference only.

Publishing research using A11250? Please let us know so that we can cite the reference in this datasheet.

Antibodies (8)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order