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[KO Validated] SQSTM1/p62 Rabbit mAb (A19700)

Publications (18) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)

Western blot analysis of extracts of various cell lines, using SQSTM1/p62 antibody (A19700) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)

Western blot analysis of extracts of PC-12 cells, using SQSTM1/p62 antibody (A19700) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)

Western blot analysis of extracts of HeLa cells, using SQSTM1/p62 antibody (A19700) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)

Western blot analysis of extracts from wild type (WT) and SQSTM1/p62 knockout (KO) 293T cells, using SQSTM1/p62 antibody (A19700) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)

Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] SQSTM1/p62 Rabbit mAb (A19700) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] SQSTM1/p62 Rabbit mAb (A19700) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)

Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug SQSTM1/p62 antibody (A19700). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (A19700) at a dilition of 1:1000.

Review
ABclonal: review for [KO Validated] SQSTM1/p62 Rabbit mAb(A19700)

Overview

Product name[KO Validated] SQSTM1/p62 Rabbit mAb
Catalog No.A19700
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 341-440 of human SQSTM1/p62 (Q13501).
SequenceLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL
Gene ID8878
Swiss protQ13501
SynonymsSQSTM1; A170; DMRV; FTDALS3; NADGP; OSIL; PDB3; ZIP3; p60; p62; p62B
Calculated MW62kDa
Observed MW62KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293T, HeLa, L929, C2C12, PC-12
Cellular locationCytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Late endosome, Lysosome, Nucleus, P-body, autophagosome
Customer validation

(HeLa、PC-3、U2-OS、293T)

WB(Mus musculus, Homo sapiens, Rattus norvegicus, Gallus gallus, Sus scrofa)

IHC(Mus musculus)

IF(Homo sapiens, Sus scrofa)

ABclonal:Western blot - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)}

Western blot - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)

Western blot analysis of extracts of various cell lines, using SQSTM1/p62 antibody (A19700) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)}

Western blot - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)

Western blot analysis of extracts of PC-12 cells, using SQSTM1/p62 antibody (A19700) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)}

Western blot - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)

Western blot analysis of extracts of HeLa cells, using SQSTM1/p62 antibody (A19700) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)}

Western blot - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)

Western blot analysis of extracts from wild type (WT) and SQSTM1/p62 knockout (KO) 293T cells, using SQSTM1/p62 antibody (A19700) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)}

Immunohistochemistry - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)

Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] SQSTM1/p62 Rabbit mAb (A19700) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)}

Immunofluorescence - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] SQSTM1/p62 Rabbit mAb (A19700) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)}

Immunoprecipitation - [KO Validated] SQSTM1/p62 Rabbit mAb (A19700)

Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug SQSTM1/p62 antibody (A19700). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (A19700) at a dilition of 1:1000.

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