Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

SQSTM1/p62 Rabbit mAb (A19700)

Review (1)Publications (55) Datasheet SDS COA

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - SQSTM1/p62 Rabbit mAb (A19700)

Western blot analysis of extracts of various cell lines, using SQSTM1/p62 antibody (A19700) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - SQSTM1/p62 Rabbit mAb (A19700)

Western blot analysis of extracts of PC-12 cells, using SQSTM1/p62 antibody (A19700) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - SQSTM1/p62 Rabbit mAb (A19700)

Western blot analysis of extracts of HeLa cells, using SQSTM1/p62 antibody (A19700) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit mAb (A19700)

Immunohistochemistry analysis of paraffin-embedded rat spleen using SQSTM1/p62 Rabbit mAb (A19700) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit mAb (A19700)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using SQSTM1/p62 Rabbit mAb (A19700) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit mAb (A19700)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using SQSTM1/p62 Rabbit mAb (A19700) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - SQSTM1/p62 Rabbit mAb (A19700)

Confocal imaging of HeLa cells using SQSTM1/p62 Rabbit mAb (A19700, dilution 1:200) (Red). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit mAb (AC049, dilution 1:100) (Green). DAPI was used for nuclear staining (Blue). Objective: 60x.

ABclonal:Immunoprecipitation - SQSTM1/p62 Rabbit mAb (A19700)

Immunoprecipitation analysis of 300 μg extracts from HeLa cells using 3 μg SQSTM1/p62 Rabbit mAb (A19700). Western blot was performed from the immunoprecipitate using [KO Validated] SQSTM1/p62 Rabbit mAb (A19700) at a dilition of 1:1000.

Review
ABclonal: review for SQSTM1/p62 Rabbit mAb(A19700)

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    Overview

    Product nameSQSTM1/p62 Rabbit mAb
    Catalog No.A19700
    Host speciesRabbit
    Purification methodAffinity purification
    IsotypeIgG
    CloneNo.ARC0180

    Background

    This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

    Immunogen information

    ImmunogenA synthetic peptide corresponding to a sequence within amino acids 341-440 of human SQSTM1/p62 (Q13501).
    SequenceLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL
    Gene ID8878
    Swiss protQ13501
    Synonymsp60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
    Calculated MW48kDa
    Observed MW62kDa

    Applications

    ReactivityHuman, Mouse, Rat
    Tested applicationsTesting results
    IF/ICCHumanMouse
    IHC-PHumanRat
    WBHumanMouseRat
    Recommended dilution
    • WB 1:500 - 1:1000
    • IHC-P 1:50 - 1:200
    • IF/ICC 1:50 - 1:200
    • IP 1:500 - 1:1000
    Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
    Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
    Positive samples293T, HeLa, L929, C2C12, PC-12
    Cellular locationCytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Late endosome, Lysosome, Nucleus, P-body, autophagosome
    Customer validation

    (HeLa、PC-3、U2-OS、293T)

    WB(Mus musculus, Homo sapiens, Rattus norvegicus, Gallus gallus, Sus scrofa, Human)

    IHC(Mus musculus)

    IF(Homo sapiens, Sus scrofa, Mus musculus)

    IF(Mus musculus,Homo sapiens)

    WB(Mus musculus,Homo sapiens)

    IP(Mus musculus)

    Documents

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    Lot number

    Research Area

    SQSTM1/p62 Rabbit mAb images

    ABclonal:Western blot - SQSTM1/p62 Rabbit mAb (A19700)}

    Western blot - SQSTM1/p62 Rabbit mAb (A19700)

    Western blot analysis of extracts of various cell lines, using SQSTM1/p62 antibody (A19700) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - SQSTM1/p62 Rabbit mAb (A19700)}

    Western blot - SQSTM1/p62 Rabbit mAb (A19700)

    Western blot analysis of extracts of PC-12 cells, using SQSTM1/p62 antibody (A19700) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - SQSTM1/p62 Rabbit mAb (A19700)}

    Western blot - SQSTM1/p62 Rabbit mAb (A19700)

    Western blot analysis of extracts of HeLa cells, using SQSTM1/p62 antibody (A19700) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit mAb (A19700)}

    Immunohistochemistry - SQSTM1/p62 Rabbit mAb (A19700)

    Immunohistochemistry analysis of paraffin-embedded rat spleen using SQSTM1/p62 Rabbit mAb (A19700) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit mAb (A19700)}

    Immunohistochemistry - SQSTM1/p62 Rabbit mAb (A19700)

    Immunohistochemistry analysis of paraffin-embedded human lung cancer using SQSTM1/p62 Rabbit mAb (A19700) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - SQSTM1/p62 Rabbit mAb (A19700)}

    Immunohistochemistry - SQSTM1/p62 Rabbit mAb (A19700)

    Immunohistochemistry analysis of paraffin-embedded mouse spleen using SQSTM1/p62 Rabbit mAb (A19700) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - SQSTM1/p62 Rabbit mAb (A19700)}

    Immunofluorescence - SQSTM1/p62 Rabbit mAb (A19700)

    Confocal imaging of HeLa cells using SQSTM1/p62 Rabbit mAb (A19700, dilution 1:200) (Red). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit mAb (AC049, dilution 1:100) (Green). DAPI was used for nuclear staining (Blue). Objective: 60x.
    ABclonal:Immunoprecipitation - SQSTM1/p62 Rabbit mAb (A19700)}

    Immunoprecipitation - SQSTM1/p62 Rabbit mAb (A19700)

    Immunoprecipitation analysis of 300 μg extracts from HeLa cells using 3 μg SQSTM1/p62 Rabbit mAb (A19700). Western blot was performed from the immunoprecipitate using [KO Validated] SQSTM1/p62 Rabbit mAb (A19700) at a dilition of 1:1000.

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    Related Targets

    Based on existing publications, the following genes are closely related to research on SQSTM1. (Numbers indicate number of published articles where both targets appear.)

    1/

    Related Research Area

    Based on existing publications, the following research topics are related to SQSTM1. (Distance between topics and target gene indicate popularity.) SQSTM1

    * Data provided by citexs.com, for reference only.

    Publishing research using A19700? Please let us know so that we can cite the reference in this datasheet.

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