Review (1)Publications (55) Datasheet SDS COA
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition
Reactivity:Human, Mouse, Rat
Western blot analysis of extracts of various cell lines, using SQSTM1/p62 antibody (A19700) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts of PC-12 cells, using SQSTM1/p62 antibody (A19700) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts of HeLa cells, using SQSTM1/p62 antibody (A19700) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded rat spleen using SQSTM1/p62 Rabbit mAb (A19700) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using SQSTM1/p62 Rabbit mAb (A19700) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse spleen using SQSTM1/p62 Rabbit mAb (A19700) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Confocal imaging of HeLa cells using SQSTM1/p62 Rabbit mAb (A19700, dilution 1:200) (Red). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit mAb (AC049, dilution 1:100) (Green). DAPI was used for nuclear staining (Blue). Objective: 60x.
Immunoprecipitation analysis of 300 μg extracts from HeLa cells using 3 μg SQSTM1/p62 Rabbit mAb (A19700). Western blot was performed from the immunoprecipitate using [KO Validated] SQSTM1/p62 Rabbit mAb (A19700) at a dilition of 1:1000.
| Product name | SQSTM1/p62 Rabbit mAb |
|---|---|
| Catalog No. | A19700 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC0180 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 341-440 of human SQSTM1/p62 (Q13501). |
|---|---|
| Sequence | LSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL |
| Gene ID | 8878 |
| Swiss prot | Q13501 |
| Synonyms | p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3 |
| Calculated MW | 48kDa |
| Observed MW | 62kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | Testing results |
| IF/ICC | |
| IHC-P | |
| WB | |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation |
| Positive samples | 293T, HeLa, L929, C2C12, PC-12 |
| Cellular location | Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Late endosome, Lysosome, Nucleus, P-body, autophagosome |
| Customer validation | (HeLa、PC-3、U2-OS、293T) WB(Mus musculus, Homo sapiens, Rattus norvegicus, Gallus gallus, Sus scrofa, Human) IHC(Mus musculus) IF(Homo sapiens, Sus scrofa, Mus musculus) IF(Mus musculus,Homo sapiens) WB(Mus musculus,Homo sapiens) IP(Mus musculus) |
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Based on existing publications, the following genes are closely related to research on SQSTM1. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to SQSTM1. (Distance between topics and target gene indicate popularity.) SQSTM1
* Data provided by citexs.com, for reference only.
Publishing research using A19700? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.
![[KO Validated] SQSTM1/p62 Rabbit pAb](https://abclonal-us.oss-us-east-1.aliyuncs.com/abclonal/Catalog/A11250/A11250_1.jpg?t=1690971986)
![[KO Validated] SQSTM1/p62 Rabbit pAb](https://abclonal-us.oss-us-east-1.aliyuncs.com/abclonal/Catalog/A21702/A21702_1.jpg?t=1693385143)
