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[KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483)
ABclonal:Western blot - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483)
ABclonal:Immunohistochemistry - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483)
ABclonal:Immunohistochemistry - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483)
ABclonal:Immunohistochemistry - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483)
ABclonal:Immunohistochemistry - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483)
ABclonal:Immunofluorescence - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483)
ABclonal:Immunoprecipitation - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483)

Overview

Product name[KO Validated] SQSTM1 / p62 Polyclonal Antibody
Catalog No.A11483
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1 / p62 (NP_001135770.1).
SequenceMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL
Gene ID8878
Swiss protQ13501
SynonymsSQSTM1; A170; DMRV; FTDALS3; NADGP; OSIL; PDB3; ZIP3; p60; p62; p62B; sequestosome-1
Calculated MW38kDa/47kDa
Observed MW62kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293T, HeLa, U-87MG
Cellular locationCytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Late endosome, Lysosome, Nucleus, P-body, autophagosome
Customer validation

WB(Rat)

ABclonal:Western blot - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483) }

Western blot - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483)

Western blot analysis of extracts of various cell lines, using SQSTM1 antibody (A11483) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483) }

Western blot - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483)

Western blot analysis of extracts from normal (control) and SQSTM1 / p62 knockout (KO) 293T cells, using SQSTM1 / p62 antibody (A11483) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483) }

Immunohistochemistry - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483)

Immunohistochemistry of paraffin-embedded human esophagus using SQSTM1 / p62 antibody (A11483) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483) }

Immunohistochemistry - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483)

Immunohistochemistry of paraffin-embedded Rat ovary using SQSTM1 / p62 antibody (A11483) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483) }

Immunohistochemistry - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483)

Immunohistochemistry of paraffin-embedded Human appendix using SQSTM1 / p62 antibody (A11483) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483) }

Immunohistochemistry - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483)

Immunohistochemistry of paraffin-embedded Mouse liver using SQSTM1 / p62 antibody (A11483) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483) }

Immunofluorescence - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483)

Immunofluorescence analysis of U2OS cells using SQSTM1 / p62 antibody (A11483) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483) }

Immunoprecipitation - [KO Validated] SQSTM1 / p62 Polyclonal Antibody (A11483)

Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug SQSTM1 / p62 antibody (A11483). Western blot was performed from the immunoprecipitate using SQSTM1 / p62 antibody (A11483) at a dilition of 1:1000.

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