Products > Catalog Antibodies > Polyclonal Antibodies

[KO Validated] SMARCE1 Rabbit pAb (A13353)

Publication (1) Datasheet

ABclonal:Western blot - [KO Validated] SMARCE1 Polyclonal Antibody (A13353)
ABclonal:Western blot - [KO Validated] SMARCE1 Polyclonal Antibody (A13353)
ABclonal:Immunofluorescence - [KO Validated] SMARCE1 Polyclonal Antibody (A13353)
ABclonal:Immunoprecipitation - [KO Validated] SMARCE1 Polyclonal Antibody (A13353)

Overview

Product name[KO Validated] SMARCE1 Rabbit pAb
Catalog No.A13353
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-411 of human SMARCE1 (NP_003070.3).
SequenceMSKRPSYAPPPTPAPATQMPSTPGFVGYNPYSHLAYNNYRLGGNPGTNSRVTASSGITIPKPPKPPDKPLMPYMRYSRKVWDQVKASNPDLKLWEIGKIIGGMWRDLTDEEKQEYLNEYEAEKIEYNESMKAYHNSPAYLAYINAKSRAEAALEEESRQRQSRMEKGEPYMSIQPAEDPDDYDDGFSMKHTATARFQRNHRLISEILSESVVPDVRSVVTTARMQVLKRQVQSLMVHQRKLEAELLQIEERHQEKKRKFLESTDSFNNELKRLCGLKVEVDMEKIAAEIAQAEEQARKRQEEREKEAAEQAERSQSSIVPEEEQAANKGEEKKDDENIPMETEETHLEETTESQQNGEEGTSTPEDKESGQEGVDSMAEEGTSDSNTGSESNSATVEEPPTDPIPEDEKKE
Gene ID6605
Swiss protQ969G3
SynonymsSMARCE1; BAF57; CSS5
Calculated MW34kDa/37kDa/39kDa/41kDa/42kDa/46kDa
Observed MW55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesU-251MG, MCF-7
Cellular locationNucleus
Customer validation

WB(Mus musculus)

ABclonal:Western blot - [KO Validated] SMARCE1 Polyclonal Antibody (A13353) }

Western blot - [KO Validated] SMARCE1 Polyclonal Antibody (A13353)

Western blot analysis of extracts of various cell lines, using SMARCE1 antibody (A13353) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] SMARCE1 Polyclonal Antibody (A13353) }

Western blot - [KO Validated] SMARCE1 Polyclonal Antibody (A13353)

Western blot analysis of extracts from normal (control) and SMARCE1 knockout (KO) HeLa cells, using SMARCE1 antibody (A13353) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Immunofluorescence - [KO Validated] SMARCE1 Polyclonal Antibody (A13353) }

Immunofluorescence - [KO Validated] SMARCE1 Polyclonal Antibody (A13353)

Immunofluorescence analysis of HeLa cells using SMARCE1 antibody (A13353).
ABclonal:Immunoprecipitation - [KO Validated] SMARCE1 Polyclonal Antibody (A13353) }

Immunoprecipitation - [KO Validated] SMARCE1 Polyclonal Antibody (A13353)

Immunoprecipitation analysis of 150ug extracts of Jurkat cells using 3ug SMARCE1 antibody (A13353). Western blot was performed from the immunoprecipitate using SMARCE1 antibody (A13353) at a dilition of 1:1000.

Inquire About This Product

Submit your question about A13353 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A13353? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
Contact us to order