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[KO Validated] SMARCC1 Rabbit pAb (A6128)

Datasheet

ABclonal:Western blot - [KO Validated] SMARCC1 Rabbit pAb (A6128)
ABclonal:Immunohistochemistry - [KO Validated] SMARCC1 Rabbit pAb (A6128)
ABclonal:Immunoprecipitation - [KO Validated] SMARCC1 Rabbit pAb (A6128)

Overview

Product name[KO Validated] SMARCC1 Rabbit pAb
Catalog No.A6128
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 80-320 of human SMARCC1 (NP_003065.3).
SequenceLVVQLLQFQEDAFGKHVTNPAFTKLPAKCFMDFKAGGALCHILGAAYKYKNEQGWRRFDLQNPSRMDRNVEMFMNIEKTLVQNNCLTRPNIYLIPDIDLKLANKLKDIIKRHQGTFTDEKSKASHHIYPYSSSQDDEEWLRPVMRKEKQVLVHWGFYPDSYDTWVHSNDVDAEIEDPPIPEKPWKVHVKWILDTDIFNEWMNEEDYEVDENRKPVSFRQRISTKNEEPVRSPERRDRKASA
Gene ID6599
Swiss protQ92922
SynonymsSMARCC1; BAF155; CRACC1; Rsc8; SRG3; SWI3
Calculated MW122kDa
Observed MW155KDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samples293T
Cellular locationNucleus
ABclonal:Western blot - [KO Validated] SMARCC1 Rabbit pAb (A6128) }

Western blot - [KO Validated] SMARCC1 Rabbit pAb (A6128)

Western blot analysis of extracts of 293T cells, using [KO Validated] SMARCC1 antibody (A6128) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - [KO Validated] SMARCC1 Rabbit pAb (A6128) }

Immunohistochemistry - [KO Validated] SMARCC1 Rabbit pAb (A6128)

Immunohistochemistry of paraffin-embedded human kidney cancer using SMARCC1 antibody (A6128) at dilution of 1:200 (40x lens).
ABclonal:Immunoprecipitation - [KO Validated] SMARCC1 Rabbit pAb (A6128) }

Immunoprecipitation - [KO Validated] SMARCC1 Rabbit pAb (A6128)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug SMARCC1 antibody (A6128). Western blot was performed from the immunoprecipitate using SMARCC1 antibody (A6128) at a dilition of 1:1000.

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