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[KO Validated] SMARCA4 Rabbit pAb (A0887)

Datasheet

ABclonal:Western blot - [KO Validated] SMARCA4 Rabbit pAb (A0887)
ABclonal:Western blot - [KO Validated] SMARCA4 Rabbit pAb (A0887)
ABclonal:Immunohistochemistry - [KO Validated] SMARCA4 Rabbit pAb (A0887)
ABclonal:Immunohistochemistry - [KO Validated] SMARCA4 Rabbit pAb (A0887)
ABclonal:Immunofluorescence - [KO Validated] SMARCA4 Rabbit pAb (A0887)

Overview

Product name[KO Validated] SMARCA4 Rabbit pAb
Catalog No.A0887
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 30-130 of human SMARCA4 (NP_003063.2).
SequencePSPGPSPGSAHSMMGPSPGPPSAGHPIPTQGPGGYPQDNMHQMHKPMESMHEKGMSDDPRYNQMKGMGMRSGGHAGMGPPPSPMDQHSQGYPSPLGGSEHA
Gene ID6597
Swiss protP51532
SynonymsBAF190; BAF190A; BRG1; CSS4; MRD16; RTPS2; SNF2; SNF2L4; SNF2LB; SWI2; hSNF2b; SMARCA4
Calculated MW181kDa/184kDa
Observed MW180kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesOVCAR3, HeLa
Cellular locationNucleus
ABclonal:Western blot - [KO Validated] SMARCA4 Rabbit pAb (A0887) }

Western blot - [KO Validated] SMARCA4 Rabbit pAb (A0887)

Western blot analysis of extracts of various cell lines, using SMARCA4 antibody (A0887) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - [KO Validated] SMARCA4 Rabbit pAb (A0887) }

Western blot - [KO Validated] SMARCA4 Rabbit pAb (A0887)

Western blot analysis of extracts from normal (control) and SMARCA4 knockout (KO) 293T cells, using SMARCA4 antibody (A0887) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - [KO Validated] SMARCA4 Rabbit pAb (A0887) }

Immunohistochemistry - [KO Validated] SMARCA4 Rabbit pAb (A0887)

Immunohistochemistry of paraffin-embedded human colon using SMARCA4 antibody (A0887) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - [KO Validated] SMARCA4 Rabbit pAb (A0887) }

Immunohistochemistry - [KO Validated] SMARCA4 Rabbit pAb (A0887)

Immunohistochemistry of paraffin-embedded human colon carcinoma using SMARCA4 antibody (A0887) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] SMARCA4 Rabbit pAb (A0887) }

Immunofluorescence - [KO Validated] SMARCA4 Rabbit pAb (A0887)

Immunofluorescence analysis of HeLa cells using SMARCA4 antibody (A0887).

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