Products > Catalog Antibodies > Polyclonal Antibodies

[KO Validated] SMAD2 Polyclonal Antibody (A7699)

Publications (4) Datasheet

ABclonal:Western blot - [KO Validated] SMAD2 Polyclonal Antibody (A7699)
ABclonal:Western blot - [KO Validated] SMAD2 Polyclonal Antibody (A7699)
ABclonal:Immunohistochemistry - [KO Validated] SMAD2 Polyclonal Antibody (A7699)
ABclonal:Immunofluorescence - [KO Validated] SMAD2 Polyclonal Antibody (A7699)
ABclonal:Immunofluorescence - [KO Validated] SMAD2 Polyclonal Antibody (A7699)
ABclonal:Immunofluorescence - [KO Validated] SMAD2 Polyclonal Antibody (A7699)

Overview

Product name[KO Validated] SMAD2 Polyclonal Antibody
Catalog No.A7699
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human SMAD2 (NP_001003652.1).
SequenceMSSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTPNTIDQWDTTG
Gene ID4087
Swiss protQ15796
SynonymsSMAD2; JV18; JV18-1; MADH2; MADR2; hMAD-2; hSMAD2; SMAD family member 2
Calculated MW48kDa/52kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesK-562, SW480, HeLa, HL-60
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Human, Mouse)

IP(Human)

IHC(Mouse)

ABclonal:Western blot - [KO Validated] SMAD2 Polyclonal Antibody (A7699) }

Western blot - [KO Validated] SMAD2 Polyclonal Antibody (A7699)

Western blot analysis of extracts of various cell lines, using SMAD2 antibody (A7699) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] SMAD2 Polyclonal Antibody (A7699) }

Western blot - [KO Validated] SMAD2 Polyclonal Antibody (A7699)

Western blot analysis of extracts from normal (control) and SMAD2 knockout (KO) HeLa cells, using SMAD2 antibody (A7699) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunohistochemistry - [KO Validated] SMAD2 Polyclonal Antibody (A7699) }

Immunohistochemistry - [KO Validated] SMAD2 Polyclonal Antibody (A7699)

Immunohistochemistry of paraffin-embedded mouse spleen using SMAD2 antibody (A7699) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] SMAD2 Polyclonal Antibody (A7699) }

Immunofluorescence - [KO Validated] SMAD2 Polyclonal Antibody (A7699)

Immunofluorescence analysis of HeLa cells using SMAD2 antibody (A7699) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] SMAD2 Polyclonal Antibody (A7699) }

Immunofluorescence - [KO Validated] SMAD2 Polyclonal Antibody (A7699)

Immunofluorescence analysis of NIH/3T3 cells using SMAD2 antibody (A7699) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] SMAD2 Polyclonal Antibody (A7699) }

Immunofluorescence - [KO Validated] SMAD2 Polyclonal Antibody (A7699)

Immunofluorescence analysis of PC-12 cells using SMAD2 antibody (A7699) at dilution of 1:100. Blue: DAPI for nuclear staining.

Inquire About This Product

Submit your question about A7699 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A7699? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
Add to Cart

or Request quote for bulk purchase

Available for next day delivery (US only)

Contact us to order