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[KO Validated] Smad2 Rabbit pAb (A7699)

Publications (6) Datasheet

ABclonal:Western blot - [KO Validated] Smad2 Rabbit pAb (A7699)

Western blot analysis of extracts of various cell lines, using Smad2 antibody (A7699) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - [KO Validated] Smad2 Rabbit pAb (A7699)

Western blot analysis of extracts from normal (control) and Smad2 knockout (KO) HeLa cells, using Smad2 antibody (A7699) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5min.

ABclonal:Immunohistochemistry - [KO Validated] Smad2 Rabbit pAb (A7699)

Immunohistochemistry of paraffin-embedded Human colon carcinoma using [KO Validated] Smad2 Rabbit pAb (A7699) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - [KO Validated] Smad2 Rabbit pAb (A7699)

Immunofluorescence analysis of HeLa cells using Smad2 antibody (A7699) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] Smad2 Rabbit pAb (A7699)

Immunofluorescence analysis of NIH/3T3 cells using Smad2 antibody (A7699) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] Smad2 Rabbit pAb (A7699)

Immunofluorescence analysis of PC-12 cells using Smad2 antibody (A7699) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product name[KO Validated] Smad2 Rabbit pAb
Catalog No.A7699
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Smad2 (NP_001003652.1).
SequenceMSSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTPNTIDQWDTTG
Gene ID4087
Swiss protQ15796
SynonymsJV18; JV18-1; MADH2; MADR2; hMAD-2; hSMAD2; Smad2; SMAD2
Calculated MW48kDa/52kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesK-562, SW480, HeLa, HL-60
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Dendrobium officinale Kimura et Migo, Homo sapiens, Mus musculus)

IP(Homo sapiens)

IHC(Mus musculus)

ABclonal:Western blot - [KO Validated] Smad2 Rabbit pAb (A7699) }

Western blot - [KO Validated] Smad2 Rabbit pAb (A7699)

Western blot analysis of extracts of various cell lines, using Smad2 antibody (A7699) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - [KO Validated] Smad2 Rabbit pAb (A7699) }

Western blot - [KO Validated] Smad2 Rabbit pAb (A7699)

Western blot analysis of extracts from normal (control) and Smad2 knockout (KO) HeLa cells, using Smad2 antibody (A7699) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5min.
ABclonal:Immunohistochemistry - [KO Validated] Smad2 Rabbit pAb (A7699) }

Immunohistochemistry - [KO Validated] Smad2 Rabbit pAb (A7699)

Immunohistochemistry of paraffin-embedded Human colon carcinoma using [KO Validated] Smad2 Rabbit pAb (A7699) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] Smad2 Rabbit pAb (A7699) }

Immunofluorescence - [KO Validated] Smad2 Rabbit pAb (A7699)

Immunofluorescence analysis of HeLa cells using Smad2 antibody (A7699) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] Smad2 Rabbit pAb (A7699) }

Immunofluorescence - [KO Validated] Smad2 Rabbit pAb (A7699)

Immunofluorescence analysis of NIH/3T3 cells using Smad2 antibody (A7699) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] Smad2 Rabbit pAb (A7699) }

Immunofluorescence - [KO Validated] Smad2 Rabbit pAb (A7699)

Immunofluorescence analysis of PC-12 cells using Smad2 antibody (A7699) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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