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[KO Validated] SIRT3 Rabbit pAb (A5718)

Publications (3) Datasheet

ABclonal:Western blot - [KO Validated] SIRT3 Rabbit pAb (A5718)
ABclonal:Western blot - [KO Validated] SIRT3 Rabbit pAb (A5718)
ABclonal:Western blot - [KO Validated] SIRT3 Rabbit pAb (A5718)
ABclonal:Immunohistochemistry - [KO Validated] SIRT3 Rabbit pAb (A5718)
ABclonal:Immunohistochemistry - [KO Validated] SIRT3 Rabbit pAb (A5718)
ABclonal:Immunohistochemistry - [KO Validated] SIRT3 Rabbit pAb (A5718)
ABclonal:Immunofluorescence - [KO Validated] SIRT3 Rabbit pAb (A5718)

Overview

Product name[KO Validated] SIRT3 Rabbit pAb
Catalog No.A5718
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human SIRT3 (NP_036371.1).
SequenceQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK
Gene ID23410
Swiss protQ9NTG7
SynonymsSIRT3; SIR2L3; sirtuin 3
Calculated MW28kDa/43kDa
Observed MW28KDa/44KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:1000
IHC 1:20 - 1:50
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, HepG2, Mouse skeletal muscle, Mouse kidney, Rat liver
Cellular locationMitochondrion matrix
Customer validation

WB(Homo sapiens, Mus musculus)

    ABclonal:Western blot - [KO Validated] SIRT3 Rabbit pAb (A5718) }

    Western blot - [KO Validated] SIRT3 Rabbit pAb (A5718)

    Western blot analysis of extracts of 293T cells, using [KO Validated] SIRT3 antibody (A5718) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - [KO Validated] SIRT3 Rabbit pAb (A5718) }

    Western blot - [KO Validated] SIRT3 Rabbit pAb (A5718)

    Western blot analysis of extracts of various cell lines, using [KO Validated] SIRT3 antibody (A5718) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - [KO Validated] SIRT3 Rabbit pAb (A5718) }

    Western blot - [KO Validated] SIRT3 Rabbit pAb (A5718)

    Western blot analysis of extracts of various cell lines, using [KO Validated] SIRT3 antibody (A5718) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - [KO Validated] SIRT3 Rabbit pAb (A5718) }

    Immunohistochemistry - [KO Validated] SIRT3 Rabbit pAb (A5718)

    Immunohistochemistry of paraffin-embedded rat kidney using SIRT3 antibody (A5718) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - [KO Validated] SIRT3 Rabbit pAb (A5718) }

    Immunohistochemistry - [KO Validated] SIRT3 Rabbit pAb (A5718)

    Immunohistochemistry of paraffin-embedded human thyroid cancer using SIRT3 antibody (A5718) at dilution of 1:100 (40x lens).
    ABclonal:Immunohistochemistry - [KO Validated] SIRT3 Rabbit pAb (A5718) }

    Immunohistochemistry - [KO Validated] SIRT3 Rabbit pAb (A5718)

    Immunohistochemistry of paraffin-embedded mouse kidney using SIRT3 antibody (A5718) at dilution of 1:100 (40x lens).
    ABclonal:Immunofluorescence - [KO Validated] SIRT3 Rabbit pAb (A5718) }

    Immunofluorescence - [KO Validated] SIRT3 Rabbit pAb (A5718)

    Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] SIRT3 Rabbit pAb (A5718) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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