Products > Catalog Antibodies > Polyclonal Antibodies

[KO Validated] SGTA Rabbit pAb (A7306)

Datasheet

ABclonal:Western blot - [KO Validated] SGTA Polyclonal Antibody (A7306)
ABclonal:Western blot - [KO Validated] SGTA Polyclonal Antibody (A7306)
ABclonal:Immunohistochemistry - [KO Validated] SGTA Polyclonal Antibody (A7306)
ABclonal:Immunofluorescence - [KO Validated] SGTA Polyclonal Antibody (A7306)

Overview

Product name[KO Validated] SGTA Rabbit pAb
Catalog No.A7306
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein which is capable of interacting with the major nonstructural protein of parvovirus H-1 and 70-kDa heat shock cognate protein; however, its function is not known. Since this transcript is expressed ubiquitously in various tissues, this protein may serve a housekeeping function.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SGTA (NP_003012.1).
SequenceMDNKKRLAYAIIQFLHDQLRHGGLSSDAQESLEVAIQCLETAFGVTVEDSDLALPQTLPEIFEAAATGKEMPQDLRSPARTPPSEEDSAEAERLKTEGNEQMKVENFEAAVHFYGKAIEL
Gene ID6449
Swiss protO43765
SynonymsSGTA; SGT; alphaSGT; hSGT
Calculated MW34kDa
Observed MW38kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, A-549, U-251MG
Cellular location
ABclonal:Western blot - [KO Validated] SGTA Polyclonal Antibody (A7306) }

Western blot - [KO Validated] SGTA Polyclonal Antibody (A7306)

Western blot analysis of extracts of various cell lines, using SGTA antibody (A7306) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] SGTA Polyclonal Antibody (A7306) }

Western blot - [KO Validated] SGTA Polyclonal Antibody (A7306)

Western blot analysis of extracts from normal (control) and SGTA knockout (KO) 293T cells, using SGTA antibody (A7306) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - [KO Validated] SGTA Polyclonal Antibody (A7306) }

Immunohistochemistry - [KO Validated] SGTA Polyclonal Antibody (A7306)

Immunohistochemistry of paraffin-embedded human liver cancer using SGTA antibody (A7306) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] SGTA Polyclonal Antibody (A7306) }

Immunofluorescence - [KO Validated] SGTA Polyclonal Antibody (A7306)

Immunofluorescence analysis of A-549 cells using SGTA antibody (A7306). Blue: DAPI for nuclear staining.

Inquire About This Product

Submit your question about A7306 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
Add to Cart

or Request quote for bulk purchase

Contact us to order