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[KO Validated] SGTA Rabbit pAb (A7306)

Datasheet

ABclonal:Western blot - [KO Validated] SGTA Polyclonal Antibody (A7306)
ABclonal:Western blot - [KO Validated] SGTA Polyclonal Antibody (A7306)
ABclonal:Immunohistochemistry - [KO Validated] SGTA Polyclonal Antibody (A7306)
ABclonal:Immunofluorescence - [KO Validated] SGTA Polyclonal Antibody (A7306)

Overview

Product name[KO Validated] SGTA Rabbit pAb
Catalog No.A7306
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein which is capable of interacting with the major nonstructural protein of parvovirus H-1 and 70-kDa heat shock cognate protein; however, its function is not known. Since this transcript is expressed ubiquitously in various tissues, this protein may serve a housekeeping function.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SGTA (NP_003012.1).
SequenceMDNKKRLAYAIIQFLHDQLRHGGLSSDAQESLEVAIQCLETAFGVTVEDSDLALPQTLPEIFEAAATGKEMPQDLRSPARTPPSEEDSAEAERLKTEGNEQMKVENFEAAVHFYGKAIEL
Gene ID6449
Swiss protO43765
SynonymsSGTA; SGT; alphaSGT; hSGT
Calculated MW34kDa
Observed MW38kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, A-549, U-251MG
Cellular location
ABclonal:Western blot - [KO Validated] SGTA Polyclonal Antibody (A7306) }

Western blot - [KO Validated] SGTA Polyclonal Antibody (A7306)

Western blot analysis of extracts of various cell lines, using SGTA antibody (A7306) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - [KO Validated] SGTA Polyclonal Antibody (A7306) }

Western blot - [KO Validated] SGTA Polyclonal Antibody (A7306)

Western blot analysis of extracts from normal (control) and SGTA knockout (KO) 293T cells, using SGTA antibody (A7306) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - [KO Validated] SGTA Polyclonal Antibody (A7306) }

Immunohistochemistry - [KO Validated] SGTA Polyclonal Antibody (A7306)

Immunohistochemistry of paraffin-embedded human liver cancer using SGTA antibody (A7306) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - [KO Validated] SGTA Polyclonal Antibody (A7306) }

Immunofluorescence - [KO Validated] SGTA Polyclonal Antibody (A7306)

Immunofluorescence analysis of A-549 cells using SGTA antibody (A7306). Blue: DAPI for nuclear staining.

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